Production and Partial Characterization of α-Amylase Enzyme from Bacillus sp. BCC 01-50 and Potential Applications
Joint Authors
Simair, Altaf Ahmed
Qureshi, Abdul Sattar
Khushk, Imrana
Ali, Chaudhry Haider
Lashari, Safia
Bhutto, Muhammad Aqeel
Mangrio, Ghulam Sughra
Lu, Changrui
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-01-12
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Amylase is an industrially important enzyme and applied in many industrial processes such as saccharification of starchy materials, food, pharmaceutical, detergent, and textile industries.
This research work deals with the optimization of fermentation conditions for α-amylase production from thermophilic bacterial strain Bacillus sp.
BCC 01-50 and characterization of crude amylase.
The time profile of bacterial growth and amylase production was investigated in synthetic medium and maximum enzyme titer was observed after 60 h.
In addition, effects of different carbon sources were tested as a substrate for amylase production and molasses was found to be the best.
Various organic and inorganic compounds, potassium nitrate, ammonium chloride, sodium nitrate, urea, yeast extract, tryptone, beef extract, and peptone, were used and beef extract was found to be the best among the nitrogen sources used.
Temperature, pH, agitation speed, and size of inoculum were also optimized.
Highest enzyme activity was obtained when the strain was cultured in molasses medium for 60 h in shaking incubator (150 rpm) at 50°C and pH 8.
Crude amylase showed maximal activity at pH 9 and 65°C.
Enzyme remained stable in alkaline pH range 9-10 and 60–70°C.
Crude amylase showed great potential for its application in detergent industry and saccharification of starchy materials.
American Psychological Association (APA)
Simair, Altaf Ahmed& Qureshi, Abdul Sattar& Khushk, Imrana& Ali, Chaudhry Haider& Lashari, Safia& Bhutto, Muhammad Aqeel…[et al.]. 2017. Production and Partial Characterization of α-Amylase Enzyme from Bacillus sp. BCC 01-50 and Potential Applications. BioMed Research International،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1139387
Modern Language Association (MLA)
Simair, Altaf Ahmed…[et al.]. Production and Partial Characterization of α-Amylase Enzyme from Bacillus sp. BCC 01-50 and Potential Applications. BioMed Research International No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1139387
American Medical Association (AMA)
Simair, Altaf Ahmed& Qureshi, Abdul Sattar& Khushk, Imrana& Ali, Chaudhry Haider& Lashari, Safia& Bhutto, Muhammad Aqeel…[et al.]. Production and Partial Characterization of α-Amylase Enzyme from Bacillus sp. BCC 01-50 and Potential Applications. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1139387
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1139387