miR-215 Inhibits Colorectal Cancer Cell Migration and Invasion via Targeting Stearoyl-CoA Desaturase
المؤلفون المشاركون
Xu, Xinhua
Ding, Yan
Yao, Jun
Wei, Zhiping
Jin, Haipeng
Chen, Chen
Feng, Jun
Ying, Rongbiao
المصدر
Computational and Mathematical Methods in Medicine
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-06-30
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Background.
This study was aimed at exploring the effects of miR-215 and its target gene stearoyl-CoA desaturase (SCD) on colorectal cancer (CRC) cell migration and invasion.
Methods.
Here, we analyzed the relationship between miR-215 and SCD, as well as the regulation of miR-215 on CRC cells.
We constructed wild-type and mutant plasmids of SCD to identify whether SCD was a target gene of miR-215 by using a luciferase reporter assay.
The expression of miR-215 and SCD was detected by quantitative real-time polymerase chain reaction (qRT-PCR) and western blot, respectively.
MTT, wound healing, and Transwell assays were applied to determine the effect of miR-215 on CRC cell proliferation, migration, and invasion.
Results.
It was found that miR-215 expression was significantly decreased in CRC tissue while SCD was highly expressed compared with those in adjacent normal tissue.
The luciferase reporter assay indicated that SCD was a direct target gene of miR-215.
Functional analysis revealed that miR-215 overexpression significantly inhibited CRC cell proliferation, migration, and invasion in vitro.
In addition, the result of rescue experiments showed that overexpression of SCD could promote the proliferation, migration, and invasion of CRC cells, and the carcinogenic effect of SCD could be inhibited by miR-215.
Conclusions.
Taken together, our findings suggested that miR-215 could inhibit CRC cell migration and invasion via targeting SCD.
The result could eventually contribute to the treatment for CRC.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Xu, Xinhua& Ding, Yan& Yao, Jun& Wei, Zhiping& Jin, Haipeng& Chen, Chen…[et al.]. 2020. miR-215 Inhibits Colorectal Cancer Cell Migration and Invasion via Targeting Stearoyl-CoA Desaturase. Computational and Mathematical Methods in Medicine،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1139492
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Xu, Xinhua…[et al.]. miR-215 Inhibits Colorectal Cancer Cell Migration and Invasion via Targeting Stearoyl-CoA Desaturase. Computational and Mathematical Methods in Medicine No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1139492
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Xu, Xinhua& Ding, Yan& Yao, Jun& Wei, Zhiping& Jin, Haipeng& Chen, Chen…[et al.]. miR-215 Inhibits Colorectal Cancer Cell Migration and Invasion via Targeting Stearoyl-CoA Desaturase. Computational and Mathematical Methods in Medicine. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1139492
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1139492
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر