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Treatment with Sildenafil and Donepezil Improves Angiogenesis in Experimentally Induced Critical Limb Ischemia
المؤلفون المشاركون
Mironiuc, Aurel I.
Gherman, Claudia D.
Constantinescu, Ioana M.
Bolfa, Pompei
Constantinescu, Dan
المصدر
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-01-24
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Objectives.
In this study, we aimed to demonstrate the role of sildenafil (an antagonist of phosphodiesterase type 5 (PDE-5)) and donepezil (a specific and reversible inhibitor of acetylcholinesterase (Ach)) in increasing ischemia-induced angiogenesis.
Method.
Critical limb ischemia was induced by ligation of the common femoral artery followed by ligation of the common iliac artery.
The operated animals were divided into 3 groups: receiving sildenafil, receiving donepezil, and surgery alone; the contralateral lower limb was used as a negative control.
The results were controlled based on clinical score and Doppler ultrasound.
Gastrocnemius muscle samples were taken from all animals, both from the ischemic and nonischemic limb and were used for histopathological and immunohistochemical examination for the evaluation of the number of nuclei/field, endothelial cells (CD31), dividing cells (Ki-67), and vascular endothelial growth factor (VEGFR-3).
Results.
An increasing tendency of the number of nuclei/field with time was observed both in the case of sildenafil and donepezil treatment.
The formation of new capillaries (the angiogenesis process) was more strongly influenced by donepezil treatment compared to sildenafil or no treatment.
This treatment significantly influenced the capillary/fiber ratio, which was increased compared to untreated ligated animals.
Sildenafil treatment led to a gradual increase in the number of dividing cells, which was significantly compared to the negative control group and compared to the ligation control group.
The same effect (increase in the number of Ki-67 positive cells) was more obvious in the case of donepezil treatment.
Conclusion.
Donepezil treatment has a better effect in ligation-induced ischemia compared to sildenafil, promoting angiogenesis in the first place, and also arteriogenesis.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Constantinescu, Ioana M.& Bolfa, Pompei& Constantinescu, Dan& Mironiuc, Aurel I.& Gherman, Claudia D.. 2017. Treatment with Sildenafil and Donepezil Improves Angiogenesis in Experimentally Induced Critical Limb Ischemia. BioMed Research International،Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1139588
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Constantinescu, Ioana M.…[et al.]. Treatment with Sildenafil and Donepezil Improves Angiogenesis in Experimentally Induced Critical Limb Ischemia. BioMed Research International No. 2017 (2017), pp.1-11.
https://search.emarefa.net/detail/BIM-1139588
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Constantinescu, Ioana M.& Bolfa, Pompei& Constantinescu, Dan& Mironiuc, Aurel I.& Gherman, Claudia D.. Treatment with Sildenafil and Donepezil Improves Angiogenesis in Experimentally Induced Critical Limb Ischemia. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1139588
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1139588
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
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