Use of Limiting Dilution Method for Isolation of Nucleus Pulposus Mesenchymal StemProgenitor Cells and Effects of Plating Density on Biological Characteristics and Plasticity
المؤلفون المشاركون
Lin, Linghan
Jia, Zhiwei
Zhao, Yachao
Wu, Yaohong
Zhao, Xiyan
Li, Yong
Guo, Ziming
Chen, Jiahai
Cheng, Shi
Wang, Deli
Ruan, Dike
المصدر
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-16، 16ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-10-08
دولة النشر
مصر
عدد الصفحات
16
التخصصات الرئيسية
الملخص EN
Objectives.
To evaluate the effects of the limiting dilution method and plating density in rat nucleus pulposus mesenchymal stem/progenitor cells (NPMSCs).
Materials and Methods.
Nucleus pulposus tissues were isolated from 12-week-old male Sprague-Dawley rats and NPMSCs were isolated using limiting dilution method.
Cells were then classified into 3 groups according to plating density.
Cell morphologies were observed, and colony-forming units, migration abilities, proliferative capacities, cell cycle percentages, multilineage differentiation capacities, stem cell biomarker expression levels, and immunophenotyping were also examined in each group.
Results.
Low density group (LD) had higher morphological homogeneity, stronger colony-forming ability, higher cell proliferation capacity, and enhanced cell migration ability relative to the other two groups (p<0.05).
Moreover, LD had more cells entering S phase, with fewer cells arrested in G0/G1 phase (p<0.05).
While all three density groups showed a multilineage differentiation potential, LD showed a higher degree of observed and semiquantified lineage specific staining (p<0.05).
Furthermore, LD displayed higher expression levels of stem cell biomarkers (Nanog, Oct4, and Sox2) and showed higher percentages of CD29+, CD44+, and CD90+ cells (p<0.05) following flow cytometry analysis.
Conclusions.
Limiting dilution method is suggested when isolating NPMSCs as a means of improving cell activity and plasticity.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Lin, Linghan& Jia, Zhiwei& Zhao, Yachao& Wu, Yaohong& Zhao, Xiyan& Li, Yong…[et al.]. 2017. Use of Limiting Dilution Method for Isolation of Nucleus Pulposus Mesenchymal StemProgenitor Cells and Effects of Plating Density on Biological Characteristics and Plasticity. BioMed Research International،Vol. 2017, no. 2017, pp.1-16.
https://search.emarefa.net/detail/BIM-1139721
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Lin, Linghan…[et al.]. Use of Limiting Dilution Method for Isolation of Nucleus Pulposus Mesenchymal StemProgenitor Cells and Effects of Plating Density on Biological Characteristics and Plasticity. BioMed Research International No. 2017 (2017), pp.1-16.
https://search.emarefa.net/detail/BIM-1139721
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Lin, Linghan& Jia, Zhiwei& Zhao, Yachao& Wu, Yaohong& Zhao, Xiyan& Li, Yong…[et al.]. Use of Limiting Dilution Method for Isolation of Nucleus Pulposus Mesenchymal StemProgenitor Cells and Effects of Plating Density on Biological Characteristics and Plasticity. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-16.
https://search.emarefa.net/detail/BIM-1139721
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1139721
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر