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MicroRNA-29b Contributes to Collagens Imbalance in Human Osteoarthritic and Dedifferentiated Articular Chondrocytes
المؤلفون المشاركون
Moulin, David
Salone, Véronique
Koufany, Meriem
Clément, Thomas
Behm-Ansmant, Isabelle
Branlant, Christiane
Charpentier, Bruno
Jouzeau, Jean-Yves
المصدر
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-05-22
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Objective.
Decreased expression of collagen type II in favour of collagen type I or X is one hallmark of chondrocyte phenotype changes in osteoarthritic (OA) cartilage.
MicroRNA- (miR-) 29b was previously shown to target collagens in several tissues.
We studied whether it could contribute to collagen imbalance in chondrocytes with an impaired phenotype.
Methods.
After preliminary microarrays screening, miR-29b levels were measured by RT- quantitative PCR in in vitro models of chondrocyte phenotype changes (IL-1β challenge or serial subculturing) and in chondrocytes from OA and non-OA patients.
Potential miR-29b targets identified in silico in 3′-UTRs of collagens mRNAs were tested with luciferase reporter assays.
The impact of premiR-29b overexpression in ATDC5 cells was studied on collagen mRNA levels and synthesis (Sirius red staining) during chondrogenesis.
Results.
MiR-29b level increased significantly in IL-1β-stimulated and weakly in subcultured chondrocytes.
A 5.8-fold increase was observed in chondrocytes from OA versus non-OA patients.
Reporter assays showed that miR-29b targeted COL2A1 and COL1A2 3′-UTRs although with a variable recovery upon mutation.
In ATDC5 cells overexpressing premiR-29b, collagen production was reduced while mRNA levels increased.
Conclusions.
By acting probably as a posttranscriptional regulator with a different efficacy on COL2A1 and COL1A2 expression, miR-29b can contribute to the collagens imbalance associated with an abnormal chondrocyte phenotype.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Moulin, David& Salone, Véronique& Koufany, Meriem& Clément, Thomas& Behm-Ansmant, Isabelle& Branlant, Christiane…[et al.]. 2017. MicroRNA-29b Contributes to Collagens Imbalance in Human Osteoarthritic and Dedifferentiated Articular Chondrocytes. BioMed Research International،Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1139726
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Moulin, David…[et al.]. MicroRNA-29b Contributes to Collagens Imbalance in Human Osteoarthritic and Dedifferentiated Articular Chondrocytes. BioMed Research International No. 2017 (2017), pp.1-12.
https://search.emarefa.net/detail/BIM-1139726
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Moulin, David& Salone, Véronique& Koufany, Meriem& Clément, Thomas& Behm-Ansmant, Isabelle& Branlant, Christiane…[et al.]. MicroRNA-29b Contributes to Collagens Imbalance in Human Osteoarthritic and Dedifferentiated Articular Chondrocytes. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1139726
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1139726
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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