The Phosphatase Inhibitor Calyculin-A Impairs Clot Retraction, Platelet Activation, and Thrombin Generation
المؤلفون المشاركون
Csernoch, László
Kappelmayer, János
Hudák, Renáta
Vincze, János
Beke Debreceni, Ildikó
Oláh, Tamás
Erdődi, Ferenc
Clemetson, Kenneth J.
المصدر
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-06-07
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
The aim of this study was to investigate the effect of the serine/threonine protein phosphatase inhibitor, calyculin-A (CLA), on clot formation and on the procoagulant activity of human platelets.
Platelet-rich plasma (PRP) samples were preincubated with buffer or CLA and subsequently platelets were activated by the protease-activated receptor 1 (PAR-1) activator, thrombin receptor activating peptide (TRAP).
Clot retraction was detected by observing clot morphology up to 1 hour, phosphatidylserine- (PS-) expression was studied by flow cytometry, and thrombin generation was measured by a fluorimetric assay.
For the intracellular Ca2+ assay, platelets were loaded with calcium-indicator dyes and the measurements were carried out using a ratiometric method with real-time confocal microscopy.
CLA preincubation inhibited clot retraction, PS-expression, and thrombin formation.
TRAP activation elicited Ca2+ response and PS-expression in a subset of platelets.
The activated PRP displayed significantly faster and enhanced thrombin generation compared to nonactivated samples.
CLA pretreatment abrogated PS-exposure and clot retraction also in TRAP-activated samples.
As a consequence of the inhibitory effect on calcium elevation and PS-expression, CLA significantly downregulated thrombin generation in PRP.
Our results show that CLA pretreatment may be a useful tool to investigate platelet activation mechanisms that contribute to clot formation and thrombin generation.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Hudák, Renáta& Vincze, János& Csernoch, László& Beke Debreceni, Ildikó& Oláh, Tamás& Erdődi, Ferenc…[et al.]. 2017. The Phosphatase Inhibitor Calyculin-A Impairs Clot Retraction, Platelet Activation, and Thrombin Generation. BioMed Research International،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1139728
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Hudák, Renáta…[et al.]. The Phosphatase Inhibitor Calyculin-A Impairs Clot Retraction, Platelet Activation, and Thrombin Generation. BioMed Research International No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1139728
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Hudák, Renáta& Vincze, János& Csernoch, László& Beke Debreceni, Ildikó& Oláh, Tamás& Erdődi, Ferenc…[et al.]. The Phosphatase Inhibitor Calyculin-A Impairs Clot Retraction, Platelet Activation, and Thrombin Generation. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1139728
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1139728
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر