Radiolabeling and Quantitative In Vivo SPECTCT Imaging Study of Liposomes Using the Novel Iminothiolane-99mTc-Tricarbonyl Complex
المؤلفون المشاركون
Varga, Zoltán
Szigeti, Krisztián
Horváth, Ildikó
Máthé, Domokos
Szigyártó, Imola Cs.
Gyurkó, István
Dóczi, Rita
المصدر
Contrast Media & Molecular Imaging
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-05-31
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
The in vivo biodistribution of liposomal formulations greatly influences the pharmacokinetics of these novel drugs; therefore the radioisotope labeling of liposomes and the use of nuclear imaging methods for in vivo studies are of great interest.
In the present work, a new procedure for the surface labeling of liposomes is presented using the novel 99mTc-tricarbonyl complex.
Liposomes mimicking the composition of two FDA approved liposomal drugs were used.
In the first step of the labeling, thiol-groups were formed on the surface of the liposomes using Traut’s reagent, which were subsequently used to bind 99mTc-tricarbonyl complex to the liposomal surface.
The labeling efficiency determined by size exclusion chromatography was 95%, and the stability of the labeled liposomes in bovine serum was found to be 94% over 2 hours.
The obtained specific activity was 50 MBq per 1 μmol lipid which falls among the highest values reported for 99mTc labeling of liposomes.
Quantitative in vivo SPECT/CT biodistribution studies revealed distinct differences between the labeled liposomes and the free 99mTc-tricarbonyl, which indicates the in vivo stability of the labeling.
As the studied liposomes were non-PEGylated, fast clearance from the blood vessels and high uptake in the liver and spleen were observed.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Varga, Zoltán& Szigyártó, Imola Cs.& Gyurkó, István& Dóczi, Rita& Horváth, Ildikó& Máthé, Domokos…[et al.]. 2017. Radiolabeling and Quantitative In Vivo SPECTCT Imaging Study of Liposomes Using the Novel Iminothiolane-99mTc-Tricarbonyl Complex. Contrast Media & Molecular Imaging،Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1141752
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Varga, Zoltán…[et al.]. Radiolabeling and Quantitative In Vivo SPECTCT Imaging Study of Liposomes Using the Novel Iminothiolane-99mTc-Tricarbonyl Complex. Contrast Media & Molecular Imaging No. 2017 (2017), pp.1-8.
https://search.emarefa.net/detail/BIM-1141752
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Varga, Zoltán& Szigyártó, Imola Cs.& Gyurkó, István& Dóczi, Rita& Horváth, Ildikó& Máthé, Domokos…[et al.]. Radiolabeling and Quantitative In Vivo SPECTCT Imaging Study of Liposomes Using the Novel Iminothiolane-99mTc-Tricarbonyl Complex. Contrast Media & Molecular Imaging. 2017. Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1141752
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1141752
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر