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Differed IL-1 Beta Response between Active TB and LTBI Cases by Ex Vivo Stimulation of Human Monocyte-Derived Macrophage with TB-Specific Antigen
المؤلفون المشاركون
Lee, Meng-Rui
Chang, Lih-Yu
Chang, Chia-Hao
Yan, Bo-Shiun
Wang, Jann-Yuan
Lin, Wan-Hsin
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-10-29
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Background.
The difference of macrophage-specific interleukin-1 beta (IL-1b) response between latent tuberculosis infection (LTBI) and active tuberculosis (TB) remains less studied.
Method.
We performed this prospective study and recruited active TB patients, contacts with LTBI, and uninfected contacts.
The gene and protein expression of human monocyte-derived macrophage (hMDM) after ex vivo stimulation by early secretory antigenic target-6KD (ESAT-6) and tuberculin purified protein derivatives (PPD) was studied by real-time PCR and flow cytometry.
The effect of caspase-1 inhibitor was also studied.
Result.
The IL-1b gene expression after 6 hr ESAT-6 1 μg/ml stimulation was different among active TB patients (n=12), LTBI cases (n=12), and uninfected contacts (n=23) (log fold change: 0.98±1.26 vs.
2.20±0.96 vs.
2.20±0.96, P=0.013).
The IL-1b gene expression at 24 hours was higher than that at 6 hours in LTBI cases (n=4) and uninfected contacts (n=6).
After 24 hr ESAT-6 1 μg/ml stimulation, the percentage of IL-1b-expressed hMDM was borderline lower in the active TB patients (n=9) than in the LTBI cases (n=10) (14.0±11.2% vs.
31.6±22.5%, P=0.065).
Compared with ESAT-6 1 μg/ml stimulation but without the addition of caspase-1 inhibitor (CasI) (55.6±16.3%), the percentage of IL-1b-positive hMDMs decreased after addition of CasI (50 μg/ml CasI: 49.8±18.2%, P=0.078; 100 μg/ml CasI: 46.6±20.8%, P=0.030; 150 μg/ml CasI: 33.7±15.5%, P=0.016).
Conclusions.
This study revealed that macrophage-specific IL-1b response differed among different stages of Mycobacterium tuberculosis infection.
The role of IL-1b and inflammasome in the process of LTBI progressing to active TB warrants further investigation.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Lee, Meng-Rui& Chang, Lih-Yu& Chang, Chia-Hao& Yan, Bo-Shiun& Wang, Jann-Yuan& Lin, Wan-Hsin. 2019. Differed IL-1 Beta Response between Active TB and LTBI Cases by Ex Vivo Stimulation of Human Monocyte-Derived Macrophage with TB-Specific Antigen. Disease Markers،Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1147800
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Lee, Meng-Rui…[et al.]. Differed IL-1 Beta Response between Active TB and LTBI Cases by Ex Vivo Stimulation of Human Monocyte-Derived Macrophage with TB-Specific Antigen. Disease Markers No. 2019 (2019), pp.1-10.
https://search.emarefa.net/detail/BIM-1147800
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Lee, Meng-Rui& Chang, Lih-Yu& Chang, Chia-Hao& Yan, Bo-Shiun& Wang, Jann-Yuan& Lin, Wan-Hsin. Differed IL-1 Beta Response between Active TB and LTBI Cases by Ex Vivo Stimulation of Human Monocyte-Derived Macrophage with TB-Specific Antigen. Disease Markers. 2019. Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1147800
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1147800
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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