Descriptive Investigation of Strongyloidiasis Infection and Characterization of Strongyloides stercoralis Using Morphological and Molecular-Based Methods
المؤلفون المشاركون
Wijerathna, Tharaka
Gunathilaka, Nayana
Chandrasena, Nilmini
Fuji, Yoshito
Gunasekara, Deepa
Gunatilaka, Ruwan Prasanna
Premaratna, Ranjan
المصدر
Case Reports in Infectious Diseases
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-08-20
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
Strongyloidiasis is caused by the nematode Strongyloides stercoralis which has the unique ability to reproduce and complete its entire life cycle within the human host through its autoinfection cycle.
Diagnosis of this infection is important because of its potential to cause fatal hyperinfection syndrome or disseminated infections in those with defective cellular immunity.
Parasitological methods based on faecal microscopy and culture often fail to detect low-intensity infections.
A multiplex polymerase chain reaction (PCR) assay was developed for the detection of S.
stercoralis, Ascaris lumbricoides, and Enterobius vermicularis by designing primers specific for the ITS1 region of ribosomal DNA of S.
stercoralis and A.
lumbricoides and 18S region of rRNA of E.
vermicularis.
A 61-year-old patient presented with chronic gastrointestinal and respiratory symptoms and weight loss with a stool microscopy positive for helminth larvae.
Stool cultures with the Harada–Mori technique yielded L3 larvae which were identified as S.
stercoralis based on morphology.
The multiplex PCR performed on DNA extracted from stool elicited the expected band at 129 bp on gel electrophoresis of the PCR yield providing molecular evidence of intestinal strongyloidiasis.
The patient’s gastrointestinal symptoms improved with a six-day course of albendazole (400 mg twice daily).
Negative posttreatment stool microscopy, culture, and PCR confirmed successful clearance of infection.
Molecular-based PCR assay is a promising tool to diagnose and assess the therapeutic efficacy of anthelmintics in intestinal helminthiases such as strongyloidiasis.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Gunathilaka, Nayana& Chandrasena, Nilmini& Wijerathna, Tharaka& Fuji, Yoshito& Gunasekara, Deepa& Gunatilaka, Ruwan Prasanna…[et al.]. 2020. Descriptive Investigation of Strongyloidiasis Infection and Characterization of Strongyloides stercoralis Using Morphological and Molecular-Based Methods. Case Reports in Infectious Diseases،Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1148169
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Gunathilaka, Nayana…[et al.]. Descriptive Investigation of Strongyloidiasis Infection and Characterization of Strongyloides stercoralis Using Morphological and Molecular-Based Methods. Case Reports in Infectious Diseases No. 2020 (2020), pp.1-7.
https://search.emarefa.net/detail/BIM-1148169
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Gunathilaka, Nayana& Chandrasena, Nilmini& Wijerathna, Tharaka& Fuji, Yoshito& Gunasekara, Deepa& Gunatilaka, Ruwan Prasanna…[et al.]. Descriptive Investigation of Strongyloidiasis Infection and Characterization of Strongyloides stercoralis Using Morphological and Molecular-Based Methods. Case Reports in Infectious Diseases. 2020. Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1148169
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1148169
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر