Huangkui Lianchang Decoction Ameliorates DSS-Induced Ulcerative Colitis in Mice by Inhibiting the NF-kappaB Signaling Pathway
المؤلفون المشاركون
He, Zongqi
Zhou, Qing
Wen, Ke
Wu, Bensheng
Sun, Xueliang
Wang, Xiaopeng
Chen, Yugen
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-04-10
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Background.
The nuclear factor kappa beta (NF-κB) signaling pathway plays an important role in ulcerative colitis (UC).
Huangkui Lianchang decoction (HLD) is an effective traditional Chinese medicinal compound used in the treatment of UC.
HLD has good effects in the clinic, but the mechanism by which HLD acts is unclear.
This study aims to reveal the exact molecular mechanism of HLD in the treatment of UC.
Methods.
Mouse ulcerative colitis was induced by dextran sulfate sodium (DSS) and treated with HLD.
Intestinal damage was assessed by disease activity index (DAI), colon macroscopic lesion scores, and histological scores.
Interleukin (IL)-6, tumor necrosis factor (TNF)-α, and IL-1β were detected in colon tissue using ELISA.
Myeloperoxidase (MPO) and superoxide dismutase (SOD) activities in the colonic mucosa were measured.
The levels of IL-6, inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2) in the colon were determined by real-time quantitative polymerase chain reaction (qPCR).
The expression of NF-κB, IκBα, and p-IκBα in the colon was measured by Western blot.
Results.
After treatment with HLD, the DAI scores, macroscopic lesion scores, and histological scores decreased, and the levels of inflammatory cytokines related to the NF-κB signaling pathway, such as IL-6, TNF-α, and IL-1β, as well as those of iNOS and COX-2, were reduced; at the same time, colonic pathological damage was alleviated, and the MPO and SOD activities decreased.
Western blot confirmed that HLD can inhibit the NF-κB signaling pathway in DSS-induced ulcerative colitis.
Conclusion.
HLD can alleviate the inflammation caused by ulcerative colitis.
In particular, high doses of HLD can significantly alleviate intestinal inflammation and have comparable efficacy to Mesalazine.
We propose that the anti-inflammatory activity of HLD on DSS-induced colitis in mice may involve the inhibition of the NF-κB pathway.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
He, Zongqi& Zhou, Qing& Wen, Ke& Wu, Bensheng& Sun, Xueliang& Wang, Xiaopeng…[et al.]. 2019. Huangkui Lianchang Decoction Ameliorates DSS-Induced Ulcerative Colitis in Mice by Inhibiting the NF-kappaB Signaling Pathway. Evidence-Based Complementary and Alternative Medicine،Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1148384
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
He, Zongqi…[et al.]. Huangkui Lianchang Decoction Ameliorates DSS-Induced Ulcerative Colitis in Mice by Inhibiting the NF-kappaB Signaling Pathway. Evidence-Based Complementary and Alternative Medicine No. 2019 (2019), pp.1-11.
https://search.emarefa.net/detail/BIM-1148384
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
He, Zongqi& Zhou, Qing& Wen, Ke& Wu, Bensheng& Sun, Xueliang& Wang, Xiaopeng…[et al.]. Huangkui Lianchang Decoction Ameliorates DSS-Induced Ulcerative Colitis in Mice by Inhibiting the NF-kappaB Signaling Pathway. Evidence-Based Complementary and Alternative Medicine. 2019. Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1148384
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1148384
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر