Evaluation of Alternative Methods to Assess the Biological Properties of Propolis on Metabolic Activity and Biofilm Formation in Streptococcus mutans
المؤلفون المشاركون
Veloz, Jorge Jesús
Alvear, Marysol
Salazar, Luis A.
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-08-18
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Several biological activities have been reported for the Chilean propolis, among their antimicrobial and antibiofilm properties, due to its high polyphenol content.
In this study, we evaluate alternative methods to assess the effect of Chilean propolis on biofilm formation and metabolic activity of Streptococcus mutans (S.
mutans), a major cariogenic agent in oral cavity.
Biofilm formation was studied by using crystal violet and by confocal microscopy.
The metabolic activity of biofilm was evaluated by MTT and by flow cytometry analysis.
The results show that propolis reduces biofilm formation and biofilm metabolic activity in S.
mutans.
When the variability of the methods to measure biofilm formation was compared, the coefficient of variation (CV) fluctuated between 12.8 and 23.1% when using crystal violet methodology.
On the other hand, the CV ranged between 2.2 and 3.3% with confocal microscopy analysis.
The CV for biofilm’s metabolic activity measured by MTT methodology ranged between 5.0 and 11.6%, in comparison with 1.9 to 3.2% when flow cytometry analysis was used.
Besides, it is possible to conclude that the methods based on colored compounds presented lower precision to study the effect of propolis on biofilm properties.
Therefore, we recommend the use of flow cytometry and confocal microscopy in S.
mutans biofilm analysis.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Veloz, Jorge Jesús& Alvear, Marysol& Salazar, Luis A.. 2019. Evaluation of Alternative Methods to Assess the Biological Properties of Propolis on Metabolic Activity and Biofilm Formation in Streptococcus mutans. Evidence-Based Complementary and Alternative Medicine،Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1148584
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Veloz, Jorge Jesús…[et al.]. Evaluation of Alternative Methods to Assess the Biological Properties of Propolis on Metabolic Activity and Biofilm Formation in Streptococcus mutans. Evidence-Based Complementary and Alternative Medicine No. 2019 (2019), pp.1-8.
https://search.emarefa.net/detail/BIM-1148584
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Veloz, Jorge Jesús& Alvear, Marysol& Salazar, Luis A.. Evaluation of Alternative Methods to Assess the Biological Properties of Propolis on Metabolic Activity and Biofilm Formation in Streptococcus mutans. Evidence-Based Complementary and Alternative Medicine. 2019. Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1148584
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1148584
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر