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A Validated HPLC-MSMS Method for Simultaneous Determination of Militarine and Its Three Metabolites in Rat Plasma: Application to a Pharmacokinetic Study
المؤلفون المشاركون
Gong, Zipeng
Li, YueTing
Huang, Yong
Sun, Hui-Yuan
Zheng, Lin
Yang, Chang
Pan, Jie
Wang, Yong-Lin
Wang, Ai-Min
Li, Yong-Jun
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-05-02
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
A rapid, reliable, and sensitive HPLC-electrospray ionization-tandem mass spectrometry (HPLC-MS/MS) method was established and validated for simultaneous determination of militarine and its three metabolites (gastrodin, α-isobutylmalic acid, and gymnoside I) in rat plasma.
Plasma was acidified with formic acid, and protein was precipitated with methanol.
MS/MS with ESI and multiple reaction monitoring at m/z 725.3→457.3, 457.1→127, 304.3→107.2, 189→129, and 417.1→267.1 was used for determination of militarine, gastrodin, α-isobutylmalic acid, gymnoside I, and puerarin (internal standard), respectively.
Chromatographic separation was conducted using an ACE UltraCore SuperC18 (2.1 × 100 mm, 2.5 μm) column with gradient mobile phase (0.1% formic acid in water and acetonitrile).
The lower limits of quantitation for militarine, gastrodin, α-isobutylmalic acid, and gymnoside I were 1.02, 2.96, 1.64, and 0.3 ng/mL, respectively.
The relative standard deviations of intra- and interday measurements were less than 15%, and the method accuracy ranged from 87.4% to 112.5%.
The extraction recovery was 83.52%-105.34%, and no matrix effect was observed.
The three metabolites (gastrodin, α-isobutylmalic acid, and gymnoside I) were synchronously detected at 0.83 h, suggesting that militarine was rapidly transformed to gastrodin, α-isobutylmalic acid, and gymnoside I.
Moreover, the area under the curve (AUC) and Cmax of militarine were significantly lower than those of gastrodin and α-isobutylmalic acid, showing that militarine was largely metabolized to gastrodin and α-isobutylmalic acid in vivo.
The studies on pharmacokinetics of militarine and its three metabolites were of great use for facilitating the clinical application of militarine and were also highly meaningful for the potential development of militarine.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Sun, Hui-Yuan& Zheng, Lin& Gong, Zipeng& Li, YueTing& Yang, Chang& Pan, Jie…[et al.]. 2019. A Validated HPLC-MSMS Method for Simultaneous Determination of Militarine and Its Three Metabolites in Rat Plasma: Application to a Pharmacokinetic Study. Evidence-Based Complementary and Alternative Medicine،Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1148917
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Sun, Hui-Yuan…[et al.]. A Validated HPLC-MSMS Method for Simultaneous Determination of Militarine and Its Three Metabolites in Rat Plasma: Application to a Pharmacokinetic Study. Evidence-Based Complementary and Alternative Medicine No. 2019 (2019), pp.1-9.
https://search.emarefa.net/detail/BIM-1148917
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Sun, Hui-Yuan& Zheng, Lin& Gong, Zipeng& Li, YueTing& Yang, Chang& Pan, Jie…[et al.]. A Validated HPLC-MSMS Method for Simultaneous Determination of Militarine and Its Three Metabolites in Rat Plasma: Application to a Pharmacokinetic Study. Evidence-Based Complementary and Alternative Medicine. 2019. Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1148917
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1148917
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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