Naomaitai Ameliorated Brain Damage in Rats with Vascular Dementia by PI3KPDK1AKT Signaling Pathway
المؤلفون المشاركون
Huang, Kui
Shen, Lei
Niu, Tieming
Zhao, Ying
Fu, Jiucun
Cao, Yunpeng
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-17، 17ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-02-05
دولة النشر
مصر
عدد الصفحات
17
التخصصات الرئيسية
الملخص EN
Background/Aims.
Naomaitai can improve blood perfusion and ameliorate the damage in the paraventricular white matter.
This study was focused on observing the neuroprotective effect of Naomaitai on the vascular dementia of rat and exploring the action mechanism of PI3K/PDK1/AKT signaling pathway.
Methods.
A vascular dementia model of rats was established by permanent, bilateral common carotid artery occlusion.
Rats’ behavior was tested by Neurological deficit score and the Morris water maze.
The pathology and apoptosis were detected through HE staining and TUNEL assay.
Myelin sheath loss and nerve fiber damage were detected by LFB staining.
Inflammatory factors, oxidative stress, and brain damage markers were detected through ELISA.
The expression of apoptosis-related proteins and PI3K/PDK1/AKT signaling pathway related proteins were measured by western blot.
The expressions of PI3K, PDK1, AKT, and MBP in paraventricular white matter cells were detected by immunofluorescence.
Results.
Naomaitai treatment decreased neurological function score in rats with vascular dementia, ameliorated paraventricular white matter damage caused by long-term hypoxia, and hypoperfusion reduced the brain injury markers S-100β and NSE contents, suppressed inflammatory reaction and oxidative stress, reduced IL-1β, IL-6, TNF-α, and MDA contents, and remarkably increased IL-10 and SOD contents.
TUNEL and western blot assay showed that Naomaitai treatment decreased neuronal cell apoptosis, increased Bcl-2 expression, and reduced caspase-3 and Bax expression.
Furthermore, we found Naomaitai inhibited PI3K and PDK1 expression and activated phosphorylated AKT protein in rats with vascular dementia.
However, the protective effect of Naomatai in rats with vascular dementia was inhibited, and expression of PI3K signaling pathway-related proteins was blocked after administration of PI3K inhibitor.
Conclusion.
Naomaitai can ameliorate brain damage in rats with vascular dementia, inhibit neuronal apoptosis, and have anti-inflammatory and antioxidative stress effects, which may be regulated by the PI3K/PDK1/AKT signaling pathway.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Huang, Kui& Shen, Lei& Niu, Tieming& Zhao, Ying& Fu, Jiucun& Cao, Yunpeng. 2019. Naomaitai Ameliorated Brain Damage in Rats with Vascular Dementia by PI3KPDK1AKT Signaling Pathway. Evidence-Based Complementary and Alternative Medicine،Vol. 2019, no. 2019, pp.1-17.
https://search.emarefa.net/detail/BIM-1149060
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Huang, Kui…[et al.]. Naomaitai Ameliorated Brain Damage in Rats with Vascular Dementia by PI3KPDK1AKT Signaling Pathway. Evidence-Based Complementary and Alternative Medicine No. 2019 (2019), pp.1-17.
https://search.emarefa.net/detail/BIM-1149060
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Huang, Kui& Shen, Lei& Niu, Tieming& Zhao, Ying& Fu, Jiucun& Cao, Yunpeng. Naomaitai Ameliorated Brain Damage in Rats with Vascular Dementia by PI3KPDK1AKT Signaling Pathway. Evidence-Based Complementary and Alternative Medicine. 2019. Vol. 2019, no. 2019, pp.1-17.
https://search.emarefa.net/detail/BIM-1149060
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1149060
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر