Susceptibility of Toxoplasma gondii to Ethanolic Extract of Tinospora crispa in Vero Cells
المؤلفون المشاركون
Wana, Mohammed Nasiru
Sharif, Alhassan Abdullahi
Unyah, Ngah Zasmy
Nordin, Norshariza
Basir, Rusliza
Alapid Ahmad, Ashraf
Mustapha, Tijjani
Majid, Roslaini Abd.
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-11-18
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Background.
Toxoplasmosis remains widely distributed globally and is one of the major neglected parasitic zoonotic infections.
The infection is still endemic in most parts of the world due to poor control as well as challenges of the currently used medications which can be overcome by using natural products.
This study evaluated the effect of ethanolic extract from the stem of Tinospora crispa (EETC) on host cell invasion and intracellular replication of Toxoplasma gondii.
Method.
The stem powder of T.
crispa was soaked in absolute ethanol for 72 hours.
The resulting ethanolic extract was screened for the presence of phytochemicals.
Vero cells monolayer in 96-well plate was infected with RH strain of T.
gondii and treated with concentrations of the EETC, Veratrine alkaloid, and clindamycin ranging from 1.56 to 200 μg/mL.
MTT assay was conducted after 24 hours to evaluate the cytotoxicity and antiparasitic activities of the EETC.
Four and 24 hours treatment models were adapted to assess the infection index and intracellular proliferation of T.
gondii in vitro.
Microscopic analysis was conducted after 24 and 48 hours exposure to EETC in both treatment models.
Results.
The study revealed that the EETC had no cytotoxic effects on Vero cells with IC50 = 179 μg/mL, as compared to clindamycin (IC50 = 116.5 μg/mL) and Veratrine alkaloid (IC50 = 60.4 μg/mL).
The EETC had good anti-toxoplasma activities with IC50 = 6.31 μg/mL in comparison with clindamycin (IC50 = 8.33 μg/mL) and Veratrine alkaloid (IC50 = 14.25 μg/mL).
The EETC caused more than 70% and 80% reduction in infection index and intracellular proliferation in both treatment models, respectively.
Conclusion.
This in vitro study showed that the EETC contains promising phytochemicals effective against T.
gondii and safe to the host cells.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Sharif, Alhassan Abdullahi& Unyah, Ngah Zasmy& Nordin, Norshariza& Basir, Rusliza& Wana, Mohammed Nasiru& Alapid Ahmad, Ashraf…[et al.]. 2019. Susceptibility of Toxoplasma gondii to Ethanolic Extract of Tinospora crispa in Vero Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1149168
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Sharif, Alhassan Abdullahi…[et al.]. Susceptibility of Toxoplasma gondii to Ethanolic Extract of Tinospora crispa in Vero Cells. Evidence-Based Complementary and Alternative Medicine No. 2019 (2019), pp.1-10.
https://search.emarefa.net/detail/BIM-1149168
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Sharif, Alhassan Abdullahi& Unyah, Ngah Zasmy& Nordin, Norshariza& Basir, Rusliza& Wana, Mohammed Nasiru& Alapid Ahmad, Ashraf…[et al.]. Susceptibility of Toxoplasma gondii to Ethanolic Extract of Tinospora crispa in Vero Cells. Evidence-Based Complementary and Alternative Medicine. 2019. Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1149168
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1149168
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر