Hydroxysafflor Yellow A Inhibits TNF-α-Induced Inflammation of Human Fetal Lung Fibroblasts via NF-κB Signaling Pathway
المؤلفون المشاركون
Liu, Sen
Wang, Yan
Wen, Huijuan
Sun, Xiaofang
Wang, Yu
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-12-26
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Objective.
Hydroxysafflor yellow A (HSYA), an effective ingredient of the Chinese herb Carthamus tinctorius L, attenuated bleomycin-induced pulmonary fibrosis in mice.
This study is to investigate the effect of HSYA on the proliferation and inflammatory level of human fetal lung fibroblasts (MRC-5 cells) induced by tumor necrosis factor-α (TNF-α) and explore the underlying mechanisms.
Methods.
MRC-5 cells were treated with different concentrations of TNF-α, HSYA, or/and etanercept (ENCP, TNF-α receptor (TNFR1) antagonist, 500 ng/mL) before cell proliferation was detected.
The laser confocal microscope was used to observe the role of HSYA in binding of TNF-α and its receptor.
Co-immunoprecipitation was used to detect the binding of TNFR1 and TAK1-TAB2 complex.
Real-time quantitative RT-PCR and western blot were used to detect the expressions of inflammation-related cytokines and proteins related with the NF-κB pathway.
Luciferase reporter gene assay and chromatin coprecipitation method were used to detect the interaction between AP-1 and TGF-β1 promoter.
Results.
TNF-α (5 ng/mL) was used to induce inflammation and proliferation in MRC-5 cells.
HSYA can partially suppress the stimulation of TNF-α on proliferation and inflammatory response of MRC-5 cells.
HSYA could compete with TNF-α to bind with TNFR1 and hamper the binding of TNFR1 to TAK1-TAB2 complex.
In addition, HSYA could also inhibit the activation of the NF-κB signal pathway and suppress the binding of TGF-β1 promoter with AP-1.
Conclusion.
Evidence in this study suggested that HSYA affects TNF-α-induced proliferation and inflammatory response of MRC-5 cells through the NF-κB/AP-1 signaling pathway, which may provide theoretical basis for HSYA treatment in pulmonary fibrosis.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Liu, Sen& Wang, Yan& Wen, Huijuan& Sun, Xiaofang& Wang, Yu. 2019. Hydroxysafflor Yellow A Inhibits TNF-α-Induced Inflammation of Human Fetal Lung Fibroblasts via NF-κB Signaling Pathway. Evidence-Based Complementary and Alternative Medicine،Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1149710
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Liu, Sen…[et al.]. Hydroxysafflor Yellow A Inhibits TNF-α-Induced Inflammation of Human Fetal Lung Fibroblasts via NF-κB Signaling Pathway. Evidence-Based Complementary and Alternative Medicine No. 2019 (2019), pp.1-9.
https://search.emarefa.net/detail/BIM-1149710
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Liu, Sen& Wang, Yan& Wen, Huijuan& Sun, Xiaofang& Wang, Yu. Hydroxysafflor Yellow A Inhibits TNF-α-Induced Inflammation of Human Fetal Lung Fibroblasts via NF-κB Signaling Pathway. Evidence-Based Complementary and Alternative Medicine. 2019. Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1149710
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1149710
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر