Hydroxysafflor Yellow A Inhibits TNF-α-Induced Inflammation of Human Fetal Lung Fibroblasts via NF-κB Signaling Pathway
Joint Authors
Liu, Sen
Wang, Yan
Wen, Huijuan
Sun, Xiaofang
Wang, Yu
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-12-26
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Objective.
Hydroxysafflor yellow A (HSYA), an effective ingredient of the Chinese herb Carthamus tinctorius L, attenuated bleomycin-induced pulmonary fibrosis in mice.
This study is to investigate the effect of HSYA on the proliferation and inflammatory level of human fetal lung fibroblasts (MRC-5 cells) induced by tumor necrosis factor-α (TNF-α) and explore the underlying mechanisms.
Methods.
MRC-5 cells were treated with different concentrations of TNF-α, HSYA, or/and etanercept (ENCP, TNF-α receptor (TNFR1) antagonist, 500 ng/mL) before cell proliferation was detected.
The laser confocal microscope was used to observe the role of HSYA in binding of TNF-α and its receptor.
Co-immunoprecipitation was used to detect the binding of TNFR1 and TAK1-TAB2 complex.
Real-time quantitative RT-PCR and western blot were used to detect the expressions of inflammation-related cytokines and proteins related with the NF-κB pathway.
Luciferase reporter gene assay and chromatin coprecipitation method were used to detect the interaction between AP-1 and TGF-β1 promoter.
Results.
TNF-α (5 ng/mL) was used to induce inflammation and proliferation in MRC-5 cells.
HSYA can partially suppress the stimulation of TNF-α on proliferation and inflammatory response of MRC-5 cells.
HSYA could compete with TNF-α to bind with TNFR1 and hamper the binding of TNFR1 to TAK1-TAB2 complex.
In addition, HSYA could also inhibit the activation of the NF-κB signal pathway and suppress the binding of TGF-β1 promoter with AP-1.
Conclusion.
Evidence in this study suggested that HSYA affects TNF-α-induced proliferation and inflammatory response of MRC-5 cells through the NF-κB/AP-1 signaling pathway, which may provide theoretical basis for HSYA treatment in pulmonary fibrosis.
American Psychological Association (APA)
Liu, Sen& Wang, Yan& Wen, Huijuan& Sun, Xiaofang& Wang, Yu. 2019. Hydroxysafflor Yellow A Inhibits TNF-α-Induced Inflammation of Human Fetal Lung Fibroblasts via NF-κB Signaling Pathway. Evidence-Based Complementary and Alternative Medicine،Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1149710
Modern Language Association (MLA)
Liu, Sen…[et al.]. Hydroxysafflor Yellow A Inhibits TNF-α-Induced Inflammation of Human Fetal Lung Fibroblasts via NF-κB Signaling Pathway. Evidence-Based Complementary and Alternative Medicine No. 2019 (2019), pp.1-9.
https://search.emarefa.net/detail/BIM-1149710
American Medical Association (AMA)
Liu, Sen& Wang, Yan& Wen, Huijuan& Sun, Xiaofang& Wang, Yu. Hydroxysafflor Yellow A Inhibits TNF-α-Induced Inflammation of Human Fetal Lung Fibroblasts via NF-κB Signaling Pathway. Evidence-Based Complementary and Alternative Medicine. 2019. Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1149710
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1149710