Study of the Binding Interaction between Wortmannin and Calf Thymus DNA: Multispectroscopic and Molecular Docking Studies
المؤلفون المشاركون
Mehran, Shiva
Rasmi, Yousef
Karamdel, Hamid Reza
Hossinzadeh, Ramin
Gholinejad, Zafar
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-12-28
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
Introduction.
Wortmannin (WTN) is a steroid metabolite that inhibits phosphatidylinositol 3-kinase and other signaling pathways.
Structurally, the WTN consists of a cyclopentanophenanthrene-like structure with several oxygen-rich moieties which have the potential to interact with deoxyribonucleic acid (DNA) molecules.
Methods.
We aim to evaluate the WTN and calf thymus DNA (ct-DNA) interaction with molecular docking using the AutoDock 4.2 software.
UV and fluorescence spectroscopy and viscosity techniques were performed to confirm the in silico analysis.
Results.
Molecular docking showed that the WTN interacted with ct-DNA via hydrogen bonds at guanine-rich sequences.
The number of hydrogen bonds between the WTN and DNA was 1-2 bonds (average 1.2) per WTN molecule.
The in silico binding constant was 2 × 103 M−1.
UV spectroscopy showed that the WTN induced a hyperchromic feature without wavelength shifting.
The WTN and DNA interaction led to quenching of DNA-emitted fluorescence.
The different concentrations of WTN had no effect on DNA viscosity.
Taken together, our results demonstrated WTN interacts with DNA in the nonintercalating mode, which is considered as a new mechanism of action.
Conclusion.
These results suggest that the WTN may exert its biological effects, at least in part, via interaction with DNA.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Mehran, Shiva& Rasmi, Yousef& Karamdel, Hamid Reza& Hossinzadeh, Ramin& Gholinejad, Zafar. 2019. Study of the Binding Interaction between Wortmannin and Calf Thymus DNA: Multispectroscopic and Molecular Docking Studies. Evidence-Based Complementary and Alternative Medicine،Vol. 2019, no. 2019, pp.1-7.
https://search.emarefa.net/detail/BIM-1149963
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Mehran, Shiva…[et al.]. Study of the Binding Interaction between Wortmannin and Calf Thymus DNA: Multispectroscopic and Molecular Docking Studies. Evidence-Based Complementary and Alternative Medicine No. 2019 (2019), pp.1-7.
https://search.emarefa.net/detail/BIM-1149963
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Mehran, Shiva& Rasmi, Yousef& Karamdel, Hamid Reza& Hossinzadeh, Ramin& Gholinejad, Zafar. Study of the Binding Interaction between Wortmannin and Calf Thymus DNA: Multispectroscopic and Molecular Docking Studies. Evidence-Based Complementary and Alternative Medicine. 2019. Vol. 2019, no. 2019, pp.1-7.
https://search.emarefa.net/detail/BIM-1149963
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1149963
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر