Determination of Electroacupuncture Effects on circRNAs in Plasma Exosomes in Diabetic Mice: An RNA-Sequencing Approach
المؤلفون المشاركون
Shou, Yin
Hu, Li
Zhang, Weibo
Gao, Yuan
Xu, Ping
Zhang, Bimeng
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-15، 15ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-09-24
دولة النشر
مصر
عدد الصفحات
15
التخصصات الرئيسية
الملخص EN
circRNAs are involved in diabetes mellitus pathogenesis.
Electroacupuncture (EA) is an effective therapeutic strategy for diabetes mellitus.
However, whether the mechanism of action of EA on diabetes mellitus is related to altered circRNAs is unclear.
The aim of this study was to reveal the effect of EA on circRNA expression in plasma exosomes and the underlying signaling pathway in mice with type 2 diabetes mellitus (T2DM).
In total, 10 mice were randomly categorized into a normal group and 20 mice were used for the T2DM model preparation and randomly divided into the model and model + EA groups.
Mice in the model + EA group were administered EA treatment.
Changes in the fasting blood glucose (FBG) level and islet structure were evaluated.
Plasma exosomes were subjected to RNA sequencing, and then bioinformatics analysis and real-time quantitative PCR (qPCR) verification were performed.
EA treatment reduced the FBG level, preserved the islet structure, and reduced the islet β cell apoptotic rate in T2DM mice.
After EA treatment, 165 differentially expressed circRNAs were found.
GO and KEGG analyses revealed that thyroid hormone signaling was actively regulated by EA.
circRNA/miRNA interaction analysis revealed mmu-mir-7092-3p to be closely associated with circINPP4B, suggesting that the phosphatidylinositol signaling pathway may be affected by EA.
qPCR confirmed that 12 circRNAs had significant differences.
These findings suggested that EA intervention can significantly protect islet function and improve the FBG level in T2DM, possibly via regulation of thyroid hormone and phosphatidylinositol signaling.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Shou, Yin& Hu, Li& Zhang, Weibo& Gao, Yuan& Xu, Ping& Zhang, Bimeng. 2019. Determination of Electroacupuncture Effects on circRNAs in Plasma Exosomes in Diabetic Mice: An RNA-Sequencing Approach. Evidence-Based Complementary and Alternative Medicine،Vol. 2019, no. 2019, pp.1-15.
https://search.emarefa.net/detail/BIM-1150963
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Shou, Yin…[et al.]. Determination of Electroacupuncture Effects on circRNAs in Plasma Exosomes in Diabetic Mice: An RNA-Sequencing Approach. Evidence-Based Complementary and Alternative Medicine No. 2019 (2019), pp.1-15.
https://search.emarefa.net/detail/BIM-1150963
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Shou, Yin& Hu, Li& Zhang, Weibo& Gao, Yuan& Xu, Ping& Zhang, Bimeng. Determination of Electroacupuncture Effects on circRNAs in Plasma Exosomes in Diabetic Mice: An RNA-Sequencing Approach. Evidence-Based Complementary and Alternative Medicine. 2019. Vol. 2019, no. 2019, pp.1-15.
https://search.emarefa.net/detail/BIM-1150963
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1150963
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر