C-Phycocyanin Alleviates Bladder Inflammation and Dysfunction in Cyclophosphamide-Induced Cystitis in a Mouse Model by Inhibiting COX-2 and EP4

الملخص EN

Objective.

To explore the effect of C-phycocyanin (C-PC) on voiding behavior and histological changes in cyclophosphamide- (CYP-) induced cystitis in mice.

Methods.

Sixty female mice were included.

The mice in the C-PC group received C-PC (25 mg/kg, twice, i.p.) and then CYP (200 mg/kg, i.p.) two hours later, while the mice in the CYP group only received the equivalent CYP.

Saline was injected in the mice in the control group.

A voided stain on paper (VSOP) test was conducted to analyze the micturition.

The bladders were harvested for histological evaluation and measurements of inflammatory factors.

Results.

C-PC reduced the micturition frequency in the mice with CYP-induced cystitis.

The bladder/body weight ratio and edema were remarkably higher in the CYP group compared to the C-PC group.

C-PC suppressed the expressions of COX-2, PGE2, and EP4 (prostaglandin E receptor 4) according to the ELISA assay.

Immunohistochemical staining also indicated that C-PC reduced the expressions of COX-2 in urothelium and EP4 in smooth muscles.

Conclusions.

C-PC relieved symptoms associated with CYP-induced cystitis in mice by inhibiting bladder inflammation through COX-2 and EP4 expression.