Effect of Jiawei Fengshining on Synovial Cell Apoptosis and TGF-β1Smad Signaling Pathway in Rats with Rheumatoid Arthritis
المؤلفون المشاركون
Dong, Xiaojun
Gan, Yuan
Ding, Lina
Zeng, Fujia
Ding, Dou
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-12-21
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Background/Aims.
Jiawei Fengshining (JWFSN) is a new formula originated from Fengshining, a classic formula for the treatment of rheumatoid arthritis (RA).
The mechanism of JWFSN in the treatment of RA is still unclear.
The aim of this study was to evaluate the effect of JWFSN formula on the inflammatory mediator levels in the serum and the TGF-β1/Smad pathway in the synovium and to explore the underlying mechanisms of JWFSN formula to ameliorate synovial hyperplasia and apoptosis inhibition of synovium in rats with RA.
Method.
SPF female Wistar rats were randomly divided into 6 groups: the blank control group, the model control group, the positive drug group, and the low-, medium-, and high- dose JWFSN groups, with 8 rats in each group.
Enzyme-linked immunosorbent assay (ELISA) was used to detect inflammatory mediators, anti-inflammatory mediators, and rheumatoid factor (RF).
The pathological condition and apoptosis of the synovial tissue were detected by hematoxylin and eosin (HE) and TUNEL staining, respectively.
TGF-β1, p-Smad2, p-Smad3, and Smad7 protein expressions in synovial tissue were measured by western blot assay.
In addition, human rheumatoid arthritis fibroblast-like synoviocytes cell line MH7A was treated with 20% JWFSN-containing serum to obtain in vitro data.
Result.
The administration of JWFSN was found to ameliorate synovial hyperplasia and promote apoptosis; increase the serum contents of anti-inflammatory mediators; reduce inflammatory mediators and RF contents; and inhibit the TGF-β1/Smad signaling pathway in CIA rats.
In vitro JWFSN treatment increased the apoptosis of MH7A cells and decreased cell viability.
Additionally, JWFSN treatment inhibited the TGF-β1/Smad signaling pathway in MH7A cells.
Interestingly, kartogenin (TGF-β1/Smad pathway activator) treament reversed the effects of JWFSN treatment.
Conclusion.
JWFSN may ameliorate inflammatory factors’ abnormality, synovial hyperplasia, and apoptosis inhibition of synovium via the TGF-β1/Smad signaling pathway.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Dong, Xiaojun& Gan, Yuan& Ding, Lina& Zeng, Fujia& Ding, Dou. 2019. Effect of Jiawei Fengshining on Synovial Cell Apoptosis and TGF-β1Smad Signaling Pathway in Rats with Rheumatoid Arthritis. Evidence-Based Complementary and Alternative Medicine،Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1151369
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Dong, Xiaojun…[et al.]. Effect of Jiawei Fengshining on Synovial Cell Apoptosis and TGF-β1Smad Signaling Pathway in Rats with Rheumatoid Arthritis. Evidence-Based Complementary and Alternative Medicine No. 2019 (2019), pp.1-12.
https://search.emarefa.net/detail/BIM-1151369
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Dong, Xiaojun& Gan, Yuan& Ding, Lina& Zeng, Fujia& Ding, Dou. Effect of Jiawei Fengshining on Synovial Cell Apoptosis and TGF-β1Smad Signaling Pathway in Rats with Rheumatoid Arthritis. Evidence-Based Complementary and Alternative Medicine. 2019. Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1151369
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1151369
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر