iTRAQ-Based Proteomics Analysis of Plasma of Myasthenia Gravis Patients Treated with Jia Wei Bu Zhong Yi Qi Decoction
المؤلفون المشاركون
Lv, Jie
Zhang, Yunke
Yang, Junhong
Chen, Yingzhe
Zhang, Jing
Zhang, Yingna
Zhao, Xue
Fang, Hua
Liu, Chongchong
Zhang, Qingyong
Cui, Xinzheng
Wang, Xiaohan
Gao, Feng
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-18، 18ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-12-13
دولة النشر
مصر
عدد الصفحات
18
التخصصات الرئيسية
الملخص EN
Myasthenia gravis (MG) is an autoimmune disease.
A proportion of MG patients did not get satisfactory results after treatment with pyridostigmine and prednisone.
Jia Wei Bu Zhong Yi Qi (Jia Wei BZYQ) decoction, a water extract from multiple herbs, has been demonstrated to be effective in the treatment of multiple “Qi deficiency type” diseases including MG in China.
In this text, we investigated protein alterations in the plasma from healthy volunteers (C), MG patients without any treatment (T1), MG patients with routine western medical treatment (T2), and MG patients with combined treatments of Jia Wei BZYQ decoction and routine western medicines (T3) and identified some potential proteins involved in the pathogenesis and treatment of MG.
iTRAQ (isobaric tags for relative and absolute quantitation) and 2D-LC-MS/MS (two-dimensional liquid chromatography-tandem mass spectrometry technologies) were employed to screen differentially expressed proteins.
The identification, quantification, functional annotation, and interaction of proteins were analyzed by matching software and databases.
In our project, 618 proteins were identified, among which 447 proteins had quantitative data.
The number of differentially expressed proteins was 110, 117, 143, 115, 86, and 158 in T1 vs.
C, T2 vs.
C, T2 vs.
T1, T3 vs.
C, T3 vs.
T1, and T3 vs.
T2 groups, respectively.
Functional annotation results showed that many differentially expressed proteins were closely associated with immune responses.
For instance, some key proteins such as C-reactive protein, apolipoprotein C-III, apolipoprotein A-II, alpha-actinin-1, and thrombospondin-1 have been found to be abnormally expressed in T3 group compared to T1 group or T2 group.
Interaction network analyses also provided some potential biomarkers or targets for MG management.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Zhang, Yunke& Yang, Junhong& Chen, Yingzhe& Lv, Jie& Zhang, Jing& Zhang, Yingna…[et al.]. 2019. iTRAQ-Based Proteomics Analysis of Plasma of Myasthenia Gravis Patients Treated with Jia Wei Bu Zhong Yi Qi Decoction. Evidence-Based Complementary and Alternative Medicine،Vol. 2019, no. 2019, pp.1-18.
https://search.emarefa.net/detail/BIM-1151582
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Zhang, Yunke…[et al.]. iTRAQ-Based Proteomics Analysis of Plasma of Myasthenia Gravis Patients Treated with Jia Wei Bu Zhong Yi Qi Decoction. Evidence-Based Complementary and Alternative Medicine No. 2019 (2019), pp.1-18.
https://search.emarefa.net/detail/BIM-1151582
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Zhang, Yunke& Yang, Junhong& Chen, Yingzhe& Lv, Jie& Zhang, Jing& Zhang, Yingna…[et al.]. iTRAQ-Based Proteomics Analysis of Plasma of Myasthenia Gravis Patients Treated with Jia Wei Bu Zhong Yi Qi Decoction. Evidence-Based Complementary and Alternative Medicine. 2019. Vol. 2019, no. 2019, pp.1-18.
https://search.emarefa.net/detail/BIM-1151582
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1151582
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر