Icariin Prevents IL-1β-Induced Apoptosis in Human Nucleus Pulposus via the PI3KAKT Pathway
المؤلفون المشاركون
Shao, Zengwu
Deng, Xiangyu
Wu, Wei
Liang, Hang
Huang, Donghua
Jing, Doudou
Zheng, Dong
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-11-12
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Purpose.
To explore the effect and possible mechanism of icariin, a prenylated flavonol glycoside derived from the Chinese herb Epimedium sagittatum that was applied to IL-1β pretreated human nucleus pulposus (NP) cells.
Methods.
Human NP cells were isolated from intervertebral discs of patients with scoliosis and lumbar spondylolisthesis.
The cells were divided into five groups: A (blank control); B (20 ng/ml IL-1β); C (20 ng/ml IL-1β + 20 μM icariin); D (20 μM icariin + 20 ng/ml IL-1β + 25 μM LY294002); E (20 ng/ml IL-1β + 25 μM LY294002).
For each of the five groups, the CCK8, apoptosis rates, ROS rates, and JC-1 rates were determined and an electron micrograph was performed.
Different expression levels of apoptosis proteins and proteins in the PI3K/AKT pathway were detected via western blot.
Results.
We found that the damage effects on human nucleus pulposus cells from 20 ng/ml of IL-1β exposure were attenuated by icariin.
When the PI3K/AKT pathway was blocked by LY294002, a specific inhibitor of this pathway, the protective effect of icariin was impaired.
In summary, icariin might be a protective traditional Chinese medicine, which prevents inflammation-induced degeneration of intervertebral discs partly through the PI3K/AKT pathway.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Deng, Xiangyu& Wu, Wei& Liang, Hang& Huang, Donghua& Jing, Doudou& Zheng, Dong…[et al.]. 2017. Icariin Prevents IL-1β-Induced Apoptosis in Human Nucleus Pulposus via the PI3KAKT Pathway. Evidence-Based Complementary and Alternative Medicine،Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1153234
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Deng, Xiangyu…[et al.]. Icariin Prevents IL-1β-Induced Apoptosis in Human Nucleus Pulposus via the PI3KAKT Pathway. Evidence-Based Complementary and Alternative Medicine No. 2017 (2017), pp.1-12.
https://search.emarefa.net/detail/BIM-1153234
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Deng, Xiangyu& Wu, Wei& Liang, Hang& Huang, Donghua& Jing, Doudou& Zheng, Dong…[et al.]. Icariin Prevents IL-1β-Induced Apoptosis in Human Nucleus Pulposus via the PI3KAKT Pathway. Evidence-Based Complementary and Alternative Medicine. 2017. Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1153234
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1153234
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر