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Rapid Screening of Potential Phosphodiesterase Inhibitors from the Roots of Ilex pubescens Hook. et Arn. Using a Combination of Ultrafiltration and LC–MS
المؤلفون المشاركون
Wang, Ling-Jun
Liu, Zichen
Lin, Zongtao
Chen, Shizhong
Xian, Shaoxiang
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-03-23
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
The cyclic nucleotide phosphodiesterase (PDE) plays an important role in regulating the levels of second messenger molecules cAMP and cGMP.
Various PDE inhibitors have been successfully developed into drugs for targeted diseases.
In addition, PDE inhibitors can also be found in different foods and natural medicines.
In this study, ultrafiltration liquid chromatography–diode-array detector–electrospray ionization–ion-trap–time-of-flight–mass spectrometry (ultrafiltration LC–DAD–ESI–IT–TOF–MS) was applied to screen PDE inhibitors from the roots of Ilex pubescens Hook.
et Arn.
As a result, 11 major compounds were identified in I.
pubescens roots, with nine compounds as potential PDE inhibitors, among which five were further confirmed to be active against PDEI and PDE5A dose-dependently in vitro, with ilexsaponin A1 and ilexsaponin B2 being the strongest.
HPLC quantification of these bioactive compounds suggested that they are major components in the plant.
The results demonstrate that ultrafiltration LC–DAD–ESI–IT–TOF–MS is an efficient method for rapid screening of PDE inhibitors from natural medicines.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Liu, Zichen& Lin, Zongtao& Chen, Shizhong& Wang, Ling-Jun& Xian, Shaoxiang. 2017. Rapid Screening of Potential Phosphodiesterase Inhibitors from the Roots of Ilex pubescens Hook. et Arn. Using a Combination of Ultrafiltration and LC–MS. Evidence-Based Complementary and Alternative Medicine،Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1153319
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Liu, Zichen…[et al.]. Rapid Screening of Potential Phosphodiesterase Inhibitors from the Roots of Ilex pubescens Hook. et Arn. Using a Combination of Ultrafiltration and LC–MS. Evidence-Based Complementary and Alternative Medicine No. 2017 (2017), pp.1-8.
https://search.emarefa.net/detail/BIM-1153319
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Liu, Zichen& Lin, Zongtao& Chen, Shizhong& Wang, Ling-Jun& Xian, Shaoxiang. Rapid Screening of Potential Phosphodiesterase Inhibitors from the Roots of Ilex pubescens Hook. et Arn. Using a Combination of Ultrafiltration and LC–MS. Evidence-Based Complementary and Alternative Medicine. 2017. Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1153319
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1153319
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
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