Knockdown of Heparanase Suppresses Invasion of Human Trophoblasts by Activating p38 MAPK Signaling Pathway
المؤلفون المشاركون
Zhou, Bin
Wang, Yan-Yun
Che, Guanglu
Gao, Linbo
Wang, Tao
Yuan, Fang
Zhang, Lin
المصدر
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-04-17
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Preeclampsia is a pregnancy-related disease with increasing maternal and perinatal morbidity and mortality worldwide.
Defective trophoblast invasion is considered to be a major factor in the pathophysiological mechanism of preeclampsia.
Heparanase, the only endo-β-glucuronidase in mammalian cells, has been shown to be abnormally expressed in the placenta of preeclampsia patients in our previous study.
The biological role and potential mechanism of heparanase in trophoblasts remain unclear.
In the present study, stably transfected HTR8/SVneo cell lines with heparanase overexpression or knockdown were constructed.
The effect of heparanase on cellular proliferation, apoptosis, invasion, tube formation, and potential pathways in trophoblasts was explored.
Our results showed that overexpression of heparanase promoted proliferation and invasion.
Knockdown of heparanase suppressed proliferation, invasion, and tube formation but induced apoptosis.
These findings reveal that downregulation of heparanase may contribute to defective placentation and plays a crucial role in the pathogenesis of preeclampsia.
Furthermore, increased activation of p38 MAPK in heparanase-knockdown HTR8/SVneo cell was shown by MAPK pathway phosphorylation array and Western blotting assay.
After pretreatment with 3 specific p38 MAPK inhibitors (BMS582949, SB203580, or BIRB796), inadequate invasion in heparanase-knockdown HTR8/SVneo cell was rescued.
That indicates that knockdown of heparanase decreases HTR8/SVneo cell invasion through excessive activation of the p38 MAPK signaling pathway.
Our study suggests that heparanase can be a potential predictive biomarker for preeclampsia at an early stage of pregnancy and represents a promising therapeutic target for the treatment of preeclampsia.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Che, Guanglu& Wang, Yan-Yun& Zhou, Bin& Gao, Linbo& Wang, Tao& Yuan, Fang…[et al.]. 2018. Knockdown of Heparanase Suppresses Invasion of Human Trophoblasts by Activating p38 MAPK Signaling Pathway. Disease Markers،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1153631
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Che, Guanglu…[et al.]. Knockdown of Heparanase Suppresses Invasion of Human Trophoblasts by Activating p38 MAPK Signaling Pathway. Disease Markers No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1153631
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Che, Guanglu& Wang, Yan-Yun& Zhou, Bin& Gao, Linbo& Wang, Tao& Yuan, Fang…[et al.]. Knockdown of Heparanase Suppresses Invasion of Human Trophoblasts by Activating p38 MAPK Signaling Pathway. Disease Markers. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1153631
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1153631
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر