![](/images/graphics-bg.png)
Identification of Protein Expression Changes in Hepatocellular Carcinoma through iTRAQ
المؤلفون المشاركون
He, Chen-chen
Zhang, Yuan-yuan
Ying, Xia
Zhao, Qian
Ma, Jinlu
Zhang, Dan
Han, Suxia
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-01-29
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Background.
Hepatocellular carcinoma (HCC) is a malignant tumor associated with a poor prognosis.
Serum biomarkers of HCC have the potential to improve the diagnosis, provide a means to monitor the tumors, and predict their malignancy.
Proteins that are expressed differentially between HCC patients and normal controls have the potential to be biomarkers.
Method.
Serum samples from 10 confirmed HCC patients and 10 controls were collected.
The differentially expressed proteins in the serum were identified using an isobaric tags for relative and absolute quantitation- (iTRAQ-) based method.
Potential serum biomarkers were validated by ELISA in another 20 HCC patients and 20 controls.
Their expression data in HCC were extracted from The Cancer Genome Atlas (TCGA) dataset.
Results.
A total of 260 proteins were measured in the serum of HCC patients and compared to those in sex- and age-matched normal controls.
Forty-one proteins displayed significant changes, with 26 being downregulated and 15 being upregulated.
Upregulated proteins included alpha-1-antitrypsin (A1AT) and peroxiredoxin 2 (PRDX2), and downregulated proteins included paraoxonase 1 (PON1) and C-reactive protein (CRP).
We then used ELISA to measure serum levels of A1AT, PRDX2, PON1, and CRP in another 20 patients with HCC and found that only PON1 levels were consistent with the iTRAQ result.
In TCGA dataset, PON1 expression was downregulated in HCC tissues (P<0.001) and low expression of PON1 was associated with poor survival in HCC patients (P<0.001).
Conclusions.
PON1 could act as a biomarker for HCC to assist in the diagnosis of HCC.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Zhang, Yuan-yuan& Ying, Xia& Zhao, Qian& Ma, Jinlu& Zhang, Dan& He, Chen-chen…[et al.]. 2020. Identification of Protein Expression Changes in Hepatocellular Carcinoma through iTRAQ. Disease Markers،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1153799
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Zhang, Yuan-yuan…[et al.]. Identification of Protein Expression Changes in Hepatocellular Carcinoma through iTRAQ. Disease Markers No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1153799
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Zhang, Yuan-yuan& Ying, Xia& Zhao, Qian& Ma, Jinlu& Zhang, Dan& He, Chen-chen…[et al.]. Identification of Protein Expression Changes in Hepatocellular Carcinoma through iTRAQ. Disease Markers. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1153799
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1153799
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
![](/images/ebook-kashef.png)
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
![](/images/kashef-image.png)