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Septin 9 Methylation in Nasopharyngeal Swabs: A Potential Minimally Invasive Biomarker for the Early Detection of Nasopharyngeal Carcinoma
المؤلفون المشاركون
Lyu, Jie-Yu
Chen, Jian-Yong
Zhang, Meng-Wen
Yu, Geng-Sheng
Zhang, Liang
Wen, Zhong
Zhang, Xiao-Jun
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-05-05
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
Nasopharyngeal carcinoma (NPC) is highly prevalent in Southeast Asia, and an unfavorable outcome is usually attributed to advanced stage NPC.
Current methods for the early diagnosis of NPC have limitations in clinical practice.
The aim of this study was to investigate the diagnostic ability of Septin 9 methylation for NPC.
A quantitative methylation-sensitive PCR (qMS-PCR) assay was developed to measure the methylation status and levels of Septin 9 in nasopharyngeal tissues and paired swabs from patients with NPC, chronic nasopharyngitis, and healthy donors.
Methylated Septin 9 was detected in 92% (23/25) of NPC tissues and 25% (4/16) of nasopharyngitis controls (p<0.05).
High-frequency hypermethylation with decreased mRNA expression of Septin 9 in NPC was also identified.
Further, Septin 9 methylation was identified in 90.5% (19/21) of NPC biopsies and 71.4% (15/21) of paired swabs, indicating a good concordance between the two sample types.
In addition, methylated Septin 9 was found in 16 (72.7%) nasal swabs from 22 NPC patients, 2 of 19 (10.5%) nasopharyngitis, but not in any of the healthy controls (p<0.01).
The methylation score in nasal swabs of the NPC group was also significantly higher than that of non-NPC controls (p<0.001).
Moreover, receiver operating characteristic (ROC) curve analysis showed an area under the curve (AUC) of 0.882 of Septin 9 methylation tests to discriminate NPC from non-NPC subjects.
Our study demonstrated that frequent methylation of Septin 9 was present in NPC.
Its detection in nasopharyngeal swabs may provide a minimally invasive and informative method for identifying early NPC cases.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Lyu, Jie-Yu& Chen, Jian-Yong& Zhang, Xiao-Jun& Zhang, Meng-Wen& Yu, Geng-Sheng& Zhang, Liang…[et al.]. 2020. Septin 9 Methylation in Nasopharyngeal Swabs: A Potential Minimally Invasive Biomarker for the Early Detection of Nasopharyngeal Carcinoma. Disease Markers،Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1153992
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Lyu, Jie-Yu…[et al.]. Septin 9 Methylation in Nasopharyngeal Swabs: A Potential Minimally Invasive Biomarker for the Early Detection of Nasopharyngeal Carcinoma. Disease Markers No. 2020 (2020), pp.1-7.
https://search.emarefa.net/detail/BIM-1153992
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Lyu, Jie-Yu& Chen, Jian-Yong& Zhang, Xiao-Jun& Zhang, Meng-Wen& Yu, Geng-Sheng& Zhang, Liang…[et al.]. Septin 9 Methylation in Nasopharyngeal Swabs: A Potential Minimally Invasive Biomarker for the Early Detection of Nasopharyngeal Carcinoma. Disease Markers. 2020. Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1153992
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1153992
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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