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Effects of Gualou Guizhi Decoction Aqueous Extract on Axonal Regeneration in Organotypic Cortical Slice Culture after Oxygen-Glucose Deprivation
المؤلفون المشاركون
Nan, Lihong
Yang, Lan
Zheng, Yanfang
He, Yibo
Xie, Qingqing
Chen, Zheming
Li, Huang
Huang, Mei
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-09-18
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Gualou Guizhi decoction (GLGZD) is effective for the clinical treatment of limb spasms caused by ischemic stroke, but its underlying mechanism is unclear.
Propidium iodide (PI) fluorescence staining, terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL), immunohistochemistry, western blot, and real-time qPCR were used to observe the axonal regeneration and neuroprotective effects of GLGZD aqueous extract on organotypic cortical slices exposed to oxygen-glucose deprivation (OGD) and further elucidate the potential mechanisms.
Compared with the OGD group, the GLGZD aqueous extract decreased the red PI fluorescence intensity; inhibited neuronal apoptosis; improved the growth of slice axons; upregulated the protein expression of tau and growth-associated protein-43; and decreased protein and mRNA expression of neurite outgrowth inhibitor protein-A (Nogo-A), Nogo receptor 1 (NgR1), ras homolog gene family A (RhoA), rho-associated coiled-coil-containing protein kinase (ROCK), and phosphorylation of collapsin response mediator protein 2 (CRMP2).
Our study found that GLGZD had a strong neuroprotective effect on brain slices after OGD injury.
GLGZD plays a vital role in promoting axonal remodeling and functional remodeling, which may be related to regulation of the expression of Nogo-A and its receptor NgR1, near the injured axons, inhibition of the Rho-ROCK pathway, and reduction of CRMP2 phosphorylation.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Nan, Lihong& Yang, Lan& Zheng, Yanfang& He, Yibo& Xie, Qingqing& Chen, Zheming…[et al.]. 2017. Effects of Gualou Guizhi Decoction Aqueous Extract on Axonal Regeneration in Organotypic Cortical Slice Culture after Oxygen-Glucose Deprivation. Evidence-Based Complementary and Alternative Medicine،Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1153997
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Nan, Lihong…[et al.]. Effects of Gualou Guizhi Decoction Aqueous Extract on Axonal Regeneration in Organotypic Cortical Slice Culture after Oxygen-Glucose Deprivation. Evidence-Based Complementary and Alternative Medicine No. 2017 (2017), pp.1-11.
https://search.emarefa.net/detail/BIM-1153997
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Nan, Lihong& Yang, Lan& Zheng, Yanfang& He, Yibo& Xie, Qingqing& Chen, Zheming…[et al.]. Effects of Gualou Guizhi Decoction Aqueous Extract on Axonal Regeneration in Organotypic Cortical Slice Culture after Oxygen-Glucose Deprivation. Evidence-Based Complementary and Alternative Medicine. 2017. Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1153997
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1153997
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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