In Silico Identification of New Targets for Diagnosis, Vaccine, and Drug Candidates against Trypanosoma cruzi
المؤلفون المشاركون
Tiwari, Sandeep
Trevisan, Rafael Obata
Santos, Malú Mateus
Desidério, Chamberttan Souza
Alves, Leandro Gomes
de Jesus Sousa, Thiago
de Castro Oliveira, Letícia
Jaiswal, Arun Kumar
Bovi, Weslley Guimarães
de Oliveira-Silva, Mariana
Costa-Madeira, Juliana Cristina
Castellano, Lúcio Roberto Cançado
Silva, Marcos Vinicius
Rodrigues Junior, Virmondes
Oliveira, Carlo José Freire
de Castro Soares, Siomar
Azevedo, Vasco
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-15، 15ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-12-10
دولة النشر
مصر
عدد الصفحات
15
التخصصات الرئيسية
الملخص EN
Chagas disease is a neglected tropical disease caused by the parasite Trypanosoma cruzi.
Despite the efforts and distinct methodologies, the search of antigens for diagnosis, vaccine, and drug targets for the disease is still needed.
The present study is aimed at identifying possible antigens that could be used for diagnosis, vaccine, and drugs targets against T.
cruzi using reverse vaccinology and molecular docking.
The genomes of 28 T.
cruzi strains available in GenBank (NCBI) were used to obtain the genomic core.
Then, subtractive genomics was carried out to identify nonhomologous genes to the host in the core.
A total of 2630 conserved proteins in 28 strains of T.
cruzi were predicted using OrthoFinder and Diamond software, in which 515 showed no homology to the human host.
These proteins were evaluated for their subcellular localization, from which 214 are cytoplasmic and 117 are secreted or present in the plasma membrane.
To identify the antigens for diagnosis and vaccine targets, we used the VaxiJen software, and 14 nonhomologous proteins were selected showing high binding efficiency with MHC I and MHC II with potential for in vitro and in vivo tests.
When these 14 nonhomologous molecules were compared against other trypanosomatids, it was found that the retrotransposon hot spot (RHS) protein is specific only for T.
cruzi parasite suggesting that it could be used for Chagas diagnosis.
Such 14 proteins were analyzed using the IEDB software to predict their epitopes in both B and T lymphocytes.
Furthermore, molecular docking analysis was performed using the software MHOLline.
As a result, we identified 6 possible T.
cruzi drug targets that could interact with 4 compounds already known as antiparasitic activities.
These 14 protein targets, along with 6 potential drug candidates, can be further validated in future studies, in vivo, regarding Chagas disease.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Trevisan, Rafael Obata& Santos, Malú Mateus& Desidério, Chamberttan Souza& Alves, Leandro Gomes& de Jesus Sousa, Thiago& de Castro Oliveira, Letícia…[et al.]. 2020. In Silico Identification of New Targets for Diagnosis, Vaccine, and Drug Candidates against Trypanosoma cruzi. Disease Markers،Vol. 2020, no. 2020, pp.1-15.
https://search.emarefa.net/detail/BIM-1154208
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Trevisan, Rafael Obata…[et al.]. In Silico Identification of New Targets for Diagnosis, Vaccine, and Drug Candidates against Trypanosoma cruzi. Disease Markers No. 2020 (2020), pp.1-15.
https://search.emarefa.net/detail/BIM-1154208
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Trevisan, Rafael Obata& Santos, Malú Mateus& Desidério, Chamberttan Souza& Alves, Leandro Gomes& de Jesus Sousa, Thiago& de Castro Oliveira, Letícia…[et al.]. In Silico Identification of New Targets for Diagnosis, Vaccine, and Drug Candidates against Trypanosoma cruzi. Disease Markers. 2020. Vol. 2020, no. 2020, pp.1-15.
https://search.emarefa.net/detail/BIM-1154208
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1154208
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر