![](/images/graphics-bg.png)
In Vitro Inhibition of α-Amylase and α-Glucosidase by Extracts from Psiadia punctulata and Meriandra bengalensis
المؤلفون المشاركون
Gebreab, Yacob Berhane
Achila, Oliver Okoth
Kidane, Yosief
Bokrezion, Temesgen
Mebrahtu, Jimmy
Mehari, Mikias
Fessehaye, Nahom
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-07-16
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Background.
This research assessed the in vitro antidiabetic activity and phytochemical constituents of the traditionally used medicinal plants, Psiadia punctulata and Meriandra bengalensis.
Method.
The leaves of both plants were subjected to cold extraction method using 70% ethanol and hot Soxhlet extraction using n-hexane, chloroform, methanol, and distilled water.
The extracts were studied for their effect on glucose transport across yeast cells and inhibition of α-amylase and α-glucosidase enzyme activities.
Thin-layer chromatographic analysis of ethanol extract was also undertaken.
Results.
The results of yeast glucose uptake assay revealed that extracts from both plants had a maximum increase in glucose uptake at the 25mM glucose concentration with a maximum dose of 2000μg/ml plant extract.
The ethanol extract of P.
punctulata and aqueous extract of M.
bengalensis showed a high activity of 68% and 96%, respectively, at 25mM and 2000μg/ml of glucose and extract concentration.
P.
punctulata exerted peak inhibition activity of α-amylase of 37.5 ± 3% mg/dl (IC50 = 0.523 mg/dl) for methanol and distilled water extract at 0.5 mg/dl, respectively.
M.
bengalensis methanol extract exhibited the highest inhibition activity of 38 ± 8 % mg/dl (IC50 = 0.543 mg/dl) at 0.5 mg/dl.
In the α-glucosidase inhibition assay, the methanolic extract of P.
punctulata exhibited the highest inhibitory activity of 17.29 ± 9% mg/dl (IC50 = 0.761 mg/dl) at 0.5mg/dl.
The chloroform extract of M.
bengalensis had the highest inhibitory activity of 30 ± 5% mg/dl (IC50=0.6mg/dl) at 0.5 mg/dL.
Phytochemical analysis of the different extracts of P.
punctulata and M.
bengalensis revealed the presence of flavonoids, alkaloids, tannins, saponins, phytosterols, and carbohydrates.
Thin-layer chromatography analysis of ethanolic extract of both plants indicated presence of 15 and 17 spots for P.
punctulata and M.
bengalensis respectively.
Conclusion.
P.
punctulata and M.
bengalensis extracts have moderate inhibitory activity against pancreatic α-amylase and relatively low inhibitory activities against α-glucosidase.
The observed effects may be associated with the presence of flavonoids, saponins, and alkaloids.
Additional in vivo analysis, toxicological studies, isolation, and structural characterization of the phytomolecules identified in this study and molecular docking studies should be undertaken.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Kidane, Yosief& Bokrezion, Temesgen& Mebrahtu, Jimmy& Mehari, Mikias& Gebreab, Yacob Berhane& Fessehaye, Nahom…[et al.]. 2018. In Vitro Inhibition of α-Amylase and α-Glucosidase by Extracts from Psiadia punctulata and Meriandra bengalensis. Evidence-Based Complementary and Alternative Medicine،Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1154548
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Kidane, Yosief…[et al.]. In Vitro Inhibition of α-Amylase and α-Glucosidase by Extracts from Psiadia punctulata and Meriandra bengalensis. Evidence-Based Complementary and Alternative Medicine No. 2018 (2018), pp.1-9.
https://search.emarefa.net/detail/BIM-1154548
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Kidane, Yosief& Bokrezion, Temesgen& Mebrahtu, Jimmy& Mehari, Mikias& Gebreab, Yacob Berhane& Fessehaye, Nahom…[et al.]. In Vitro Inhibition of α-Amylase and α-Glucosidase by Extracts from Psiadia punctulata and Meriandra bengalensis. Evidence-Based Complementary and Alternative Medicine. 2018. Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1154548
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1154548
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
![](/images/ebook-kashef.png)
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
![](/images/kashef-image.png)