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Xingnaojing Injection Protects against Cerebral Ischemia Reperfusion Injury via PI3KAkt-Mediated eNOS Phosphorylation
المؤلفون المشاركون
Tao, Lina
Qu, Xiaoyu
Zhang, Si-xi
Zhang, Yueming
Gao, Huan
Zhai, Jing-Hui
Song, Yan-Qing
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-08-08
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
Xingnaojing (XNJ) injection, derived from traditional Chinese medicine formulation, has a protective effect against stroke, but the underlying mechanism is unclear, which severely limited its clinical application.
This research aims to elucidate the role and mechanism of XNJ in reducing cerebral ischemic reperfusion (I/R) injury.
Rats received 2 h cerebral ischemia followed by reperfusion of 24 h and were intraperitoneally given 5, 10, or 15 ml/kg XNJ 24 h before ischemia and at the onset of reperfusion, respectively.
TTC staining, HE staining, and neurological score were implied to evaluate the effectiveness of XNJ.
The protein expressions of PI3K/Akt and eNOS signaling were measured.
Experiments were further performed in human brain microvascular endothelial cells (HBMECs) to investigate the protective mechanisms of XNJ.
HBMECs were subjected to 3 h oxygen and glucose deprivation following 24 h of reoxygenation (OGD) to mimic cerebral I/R in vitro.
PI3K inhibitor LY294002 was added with or without the preconditioning of XNJ.
Multiple methods including western blot, immunofluorescence, DAPI staining, JC-1, and flow cytometry were carried out to evaluate the effect of XNJ on HBMECs.
XNJ could improve rat cerebral ischemic injury and OGD induced HBMECs apoptosis.
In vivo and in vitro researches indicated that the mechanism might be relevant to the activation of PI3K/Akt/eNOS signaling.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Zhang, Yueming& Qu, Xiaoyu& Zhai, Jing-Hui& Tao, Lina& Gao, Huan& Song, Yan-Qing…[et al.]. 2018. Xingnaojing Injection Protects against Cerebral Ischemia Reperfusion Injury via PI3KAkt-Mediated eNOS Phosphorylation. Evidence-Based Complementary and Alternative Medicine،Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1154573
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Zhang, Yueming…[et al.]. Xingnaojing Injection Protects against Cerebral Ischemia Reperfusion Injury via PI3KAkt-Mediated eNOS Phosphorylation. Evidence-Based Complementary and Alternative Medicine No. 2018 (2018), pp.1-13.
https://search.emarefa.net/detail/BIM-1154573
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Zhang, Yueming& Qu, Xiaoyu& Zhai, Jing-Hui& Tao, Lina& Gao, Huan& Song, Yan-Qing…[et al.]. Xingnaojing Injection Protects against Cerebral Ischemia Reperfusion Injury via PI3KAkt-Mediated eNOS Phosphorylation. Evidence-Based Complementary and Alternative Medicine. 2018. Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1154573
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1154573
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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