Bushenhuoxue Formula Facilitates Articular Cartilage Repair and Attenuates Matrix Degradation by Activation of TGF-β Signaling Pathway
المؤلفون المشاركون
Jin, Hongting
Xu, Taotao
Wang, Pinger
Dong, Rui
Ying, Jun
Hu, Songfeng
Zhang, Peng
Xia, Chenjie
Fang, Liang
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-10-08
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Objective.
To investigate the effect and underlying mechanism of Bushenhuoxue (BSHX) formula on articular cartilage repair.
Methods.
Twenty-four full-thickness cartilage defect rats were divided into two groups: model group and BSHX group (treated with BSHX formula).
Macroscopic observation and histopathological study were conducted after 4- and 8-week treatment.
Additionally, we also evaluated chondrocyte proliferation, extracellular matrix (ECM) deposition, cartilage degradation, and chondrocyte hypertrophy-related genes expression in chondrogenic ATDC5 cells cultured in BSHX formula-mediated serum.
Moreover, we assessed aforementioned genes expression and pSMAD2/3 protein level in Tgfβr2 siRNA transfected chondrogenic ATDC5 cells in order to address whether BSHX formula exerts cartilage repairing effect through TGF-β signaling.
Results.
Neocartilage regeneration promotion effect was observed in cartilage defect rats after BSHX formula treatment, with increases in Col2 and pSMAD2 and decreases in Mmp13 and Runx2.
Moreover, cell proliferation, the elevated Col2a1, Aggrecan and pSMAD2/3, reduced Mmp13, Adamts5, Col10a1, and Runx2 expression were also observed in chondrogenic ATDC5 cells cultured in BSHX formula-mediated serum.
Besides, the expression alteration of ECM deposition, cartilage degradation, chondrocyte hypertrophy-related genes, and pSMAD2/3 protein levels presented in Tgfβr2 downregulated chondrogenic ATDC5 cells couldn’t be adjusted by BSHX formula treatment.
Conclusion.
By activation of TGF-β signaling, BSHX formula can promote articular cartilage repair by accelerating chondrocyte proliferation and maintaining chondrocyte phenotype, upregulate ECM accumulation, and inhibit matrix degradation.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Dong, Rui& Ying, Jun& Xu, Taotao& Hu, Songfeng& Zhang, Peng& Xia, Chenjie…[et al.]. 2018. Bushenhuoxue Formula Facilitates Articular Cartilage Repair and Attenuates Matrix Degradation by Activation of TGF-β Signaling Pathway. Evidence-Based Complementary and Alternative Medicine،Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1154658
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Dong, Rui…[et al.]. Bushenhuoxue Formula Facilitates Articular Cartilage Repair and Attenuates Matrix Degradation by Activation of TGF-β Signaling Pathway. Evidence-Based Complementary and Alternative Medicine No. 2018 (2018), pp.1-11.
https://search.emarefa.net/detail/BIM-1154658
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Dong, Rui& Ying, Jun& Xu, Taotao& Hu, Songfeng& Zhang, Peng& Xia, Chenjie…[et al.]. Bushenhuoxue Formula Facilitates Articular Cartilage Repair and Attenuates Matrix Degradation by Activation of TGF-β Signaling Pathway. Evidence-Based Complementary and Alternative Medicine. 2018. Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1154658
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1154658
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر