Andrographolide Inhibits Inflammatory Cytokines Secretion in LPS-Stimulated RAW264.7 Cells through Suppression of NF-κBMAPK Signaling Pathway
المؤلفون المشاركون
Li, Yu
He, Shengnan
Tang, Jishun
Ding, Nana
Chu, Xiaoyan
Cheng, Lianping
Ding, Xuedong
Liang, Ting
Feng, Shibin
Rahman, Sajid Ur
Wang, Xichun
Wu, Jinjie
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-06-06
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Andrographolide, the main active component extracted from Andrographis paniculata (Burm.f.) Wall.
ex Nees, exerts anti-inflammatory effects; however, the principal molecular mechanisms remain unclear.
The objective of this study was to investigate the molecular mechanisms of Andrographolide in modifying lipopolysaccharide- (LPS-) induced signaling pathway in RAW264.7 cells.
An in vitro model of inflammation was induced by LPS in mouse RAW264.7 cells in the presence of Andrographolide.
The concentration and expression levels of proinflammatory cytokines were determined by an enzyme-linked immunosorbent assay (ELISA) and quantitative real-time polymerase chain reaction (qRT-PCR), respectively.
The nuclear level of NF-κB was measured by an electrophoretic mobility shift assay (EMSA).
The expression levels of NF-κB, p38, ERK, and JNK were determined by western blot.
Andrographolide dose-dependently inhibited the release and mRNA expression of TNF-α, IL-6, and IL-1β in LPS-stimulated RAW264.7 cells.
The nuclear level of p65 protein was decreased in Andrographolide treatment group.
Western blot analysis showed that Andrographolide suppressed LPS-induced NF-κB activation and the phosphorylation of IkBa, ERK1/2, JNK, and p38.
These results suggest that Andrographolide exerts an anti-inflammatory effect by inhibiting the activation of NF-κB/MAPK signaling pathway and the induction of proinflammatory cytokines.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Li, Yu& He, Shengnan& Tang, Jishun& Ding, Nana& Chu, Xiaoyan& Cheng, Lianping…[et al.]. 2017. Andrographolide Inhibits Inflammatory Cytokines Secretion in LPS-Stimulated RAW264.7 Cells through Suppression of NF-κBMAPK Signaling Pathway. Evidence-Based Complementary and Alternative Medicine،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1154699
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Li, Yu…[et al.]. Andrographolide Inhibits Inflammatory Cytokines Secretion in LPS-Stimulated RAW264.7 Cells through Suppression of NF-κBMAPK Signaling Pathway. Evidence-Based Complementary and Alternative Medicine No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1154699
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Li, Yu& He, Shengnan& Tang, Jishun& Ding, Nana& Chu, Xiaoyan& Cheng, Lianping…[et al.]. Andrographolide Inhibits Inflammatory Cytokines Secretion in LPS-Stimulated RAW264.7 Cells through Suppression of NF-κBMAPK Signaling Pathway. Evidence-Based Complementary and Alternative Medicine. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1154699
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1154699
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر