In Vitro Evaluation of the Neuroprotective Effect of Panax notoginseng Saponins by Activating the EGFRPI3KAKT Pathway
المؤلفون المشاركون
Zou, Yihuai
Gao, Yonghong
Zhao, Yi-Zhou
Sun, Yikun
Wu, Shuang
Yang, Tiantian
Cen, Kai
Shi, Xiaowei
Zhou, Dongrui
Zhu, Lingqun
Chai, Limin
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-07-31
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Context.
About 15 million people worldwide suffer strokes each year and 5 million people are left with permanent disabilities which is due to the loss of local blood supply to the brain, resulting in a neurologic deficit.
Panax notoginseng (Bruk.) F.
H.
Chen (Araliaceae) is a traditional Chinese herbal medicine widely used in the treatment of cardio-cerebrovascular diseases.
Objective.
This study investigated whether Panax notoginseng saponins (PNS) extracted from Panax notoginseng (Bruk.) F.
H.
Chen played a neuroprotective role by affecting the EGFR/PI3K/AKT pathway in oxygen-glucose deprived (OGD) SH-SY5Y cells.
Materials and Methods.
Different groups of OGD SH-SY5Y cells were treated with varying doses of PNS, PNS + AG1478 (a specific inhibitor of EGFR), or AG1478 for 16 hours.
CCK8, Annexin V-FITC/PI apoptosis analysis, and LDH release analysis were used to determine cell viability, apoptosis rate, and amounts of LDH.
Quantitative real-time PCR (q-RT-PCR) and western blotting were used to measure mRNA and proteins levels of p-EGFR/EGFR, p-PI3K/PI3K, and p-AKT/AKT in SH-SY5Y cells subjected to OGD.
Results.
PNS significantly enhanced cell viability, reduced apoptosis, and weakened cytotoxicity by inhibiting the release of LDH.
The mRNA expression profiles of EGFR, PI3K, and AKT showed no difference between model and other groups.
Additionally, ratios of p-EGFR, p-PI3K, and p-AKT to EGFR, PI3K, and AKT proteins expression, respectively, all increased significantly.
Conclusions.
These findings indicate that PNS enhanced neuroprotective effects by activating the EGFR/PI3K/AKT pathway and elevating phosphorylation levels in OGD SH-SY5Y cells.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Wu, Shuang& Yang, Tiantian& Cen, Kai& Zou, Yihuai& Shi, Xiaowei& Zhou, Dongrui…[et al.]. 2020. In Vitro Evaluation of the Neuroprotective Effect of Panax notoginseng Saponins by Activating the EGFRPI3KAKT Pathway. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1154795
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Wu, Shuang…[et al.]. In Vitro Evaluation of the Neuroprotective Effect of Panax notoginseng Saponins by Activating the EGFRPI3KAKT Pathway. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-11.
https://search.emarefa.net/detail/BIM-1154795
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Wu, Shuang& Yang, Tiantian& Cen, Kai& Zou, Yihuai& Shi, Xiaowei& Zhou, Dongrui…[et al.]. In Vitro Evaluation of the Neuroprotective Effect of Panax notoginseng Saponins by Activating the EGFRPI3KAKT Pathway. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1154795
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1154795
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر