Development and Application of an UHPLC-MSMS Method for Comparative Pharmacokinetic Study of Eight Major Bioactive Components from Yin Chen Hao Tang in Normal and Acute Liver Injured Rats
المؤلفون المشاركون
Chai, Yifeng
Zhu, Zhen-yu
Zhao, Liang
Sun, Sen
Wang, Yun
Xing, Xinrui
Cao, Yan
Chen, Yang
Chen, Si
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-11-01
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Yin Chen Hao Tang (YCHT) is one of the most famous hepatoprotective herbal formulas in China, but its pharmacokinetic investigation in model rats has been rarely conducted.
In this study, the hepatic injury model was caused by intraperitoneal injections of carbon tetrachloride (CCl4), and YCHT was orally administered to the model and normal rats.
An ultrahigh performance liquid chromatography–tandem mass spectrometry (UHPLC–MS/MS) method was established to analyze the plasma pharmacokinetics of eight major bioactive ingredients from YCHT in both the normal and liver injured rats.
The calibration curves presented good linearity (r > 0.9981) in the concentration range.
The relative standard deviation (RSD%) of inter- and intraday precision was within 9.55%, and the accuracy (RE%) ranged from -10.72% to 2.46%.
The extraction recovery, matrix effect, and stability were demonstrated to be within acceptable ranges.
The lower limit of detection (LLOD) and lower limit of quantitation (LLOQ) were around 0.1 ng/mL and 0.5 ng/mL, respectively, which were much lower than those in other related researches.
Results reveal that there are significant differences in the pharmacokinetics of scoparone, geniposide, rhein, aloe-emodin, physcion, and chrysophanol in hepatic injured rats as compared to those in control except for scopoletin and emodin.
Our experimental results provide a meaningful reference for the clinical dosage of YCHT in treating liver disorders, and the improvement of LLOD and LLOQ can also broaden the range of our method’s application, which is very suitable for quantitating these eight compounds with low levels.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Wang, Yun& Xing, Xinrui& Cao, Yan& Zhao, Liang& Sun, Sen& Chen, Yang…[et al.]. 2018. Development and Application of an UHPLC-MSMS Method for Comparative Pharmacokinetic Study of Eight Major Bioactive Components from Yin Chen Hao Tang in Normal and Acute Liver Injured Rats. Evidence-Based Complementary and Alternative Medicine،Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1154812
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Wang, Yun…[et al.]. Development and Application of an UHPLC-MSMS Method for Comparative Pharmacokinetic Study of Eight Major Bioactive Components from Yin Chen Hao Tang in Normal and Acute Liver Injured Rats. Evidence-Based Complementary and Alternative Medicine No. 2018 (2018), pp.1-12.
https://search.emarefa.net/detail/BIM-1154812
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Wang, Yun& Xing, Xinrui& Cao, Yan& Zhao, Liang& Sun, Sen& Chen, Yang…[et al.]. Development and Application of an UHPLC-MSMS Method for Comparative Pharmacokinetic Study of Eight Major Bioactive Components from Yin Chen Hao Tang in Normal and Acute Liver Injured Rats. Evidence-Based Complementary and Alternative Medicine. 2018. Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1154812
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1154812
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر