Chinese Herbal Formula, Huayu Tongbi Fang, Attenuates Inflammatory Proliferation of Rat Synoviocytes Induced by IL-1β by Regulating Proliferation and Differentiation of T Lymphocytes
المؤلفون المشاركون
Li, Jinyu
Huang, Qing-chun
Li, Xue
Zhou, Jingwei
Chen, Hening
Wang, Changzhi
Huandike, Meiyier
Liu, Juan
Zhang, Huijie
Chai, Limin
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-18، 18ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-05-20
دولة النشر
مصر
عدد الصفحات
18
التخصصات الرئيسية
الملخص EN
The inflammatory proliferation of fibroblast-like synoviocytes (FLSs) and functional imbalances in T lymphocytes play critical roles in the pathogenesis of rheumatoid arthritis (RA).
The clinical efficacy of Huayu Tongbi Fang (HYTB, a traditional herbal formula) in RA treatment has been validated.
In this study, we aimed to explore the regulatory mechanisms of HYTB on the proliferation and differentiation of T lymphocytes, and the inhibitory effect of HYTB on inflammatory proliferation of FLSs.
The RCS-364 (Rat FLSs) cells were cocultured with rat splenic lymphocytes that were induced by interleukin-1β in Transwell chambers.
After freeze-dried HYTB powder treatment, the percentage of T-cell subset and apoptosis rates of FLSs were measured using flow cytometry.
Furthermore, protein expression of key molecules of NF-κB and JAK/STAT signaling pathways was quantified using Western blot.
The granulocyte-macrophage colony-stimulating factor (GM-CSF) was measured using enzyme-linked immunosorbent assay.
The results showed that HYTB could inhibit the inflammatory proliferation of FLSs through inducing cell apoptosis.
Additionally, HYTB treatment could intervene in the proliferation and differentiation of T lymphocytes and regulate protein expression of key molecules in NF-κB and JAK/STAT cell signaling pathways.
Moreover, it could inhibit FLS activation by suppressing GM-CSF production by T cells and FLSs.
Therefore, the HYTB formula should be used as a traditional medicine against RA in modern complementary and alternative therapies.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Chen, Hening& Wang, Changzhi& Li, Jinyu& Huandike, Meiyier& Liu, Juan& Huang, Qing-chun…[et al.]. 2020. Chinese Herbal Formula, Huayu Tongbi Fang, Attenuates Inflammatory Proliferation of Rat Synoviocytes Induced by IL-1β by Regulating Proliferation and Differentiation of T Lymphocytes. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-18.
https://search.emarefa.net/detail/BIM-1154919
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Chen, Hening…[et al.]. Chinese Herbal Formula, Huayu Tongbi Fang, Attenuates Inflammatory Proliferation of Rat Synoviocytes Induced by IL-1β by Regulating Proliferation and Differentiation of T Lymphocytes. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-18.
https://search.emarefa.net/detail/BIM-1154919
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Chen, Hening& Wang, Changzhi& Li, Jinyu& Huandike, Meiyier& Liu, Juan& Huang, Qing-chun…[et al.]. Chinese Herbal Formula, Huayu Tongbi Fang, Attenuates Inflammatory Proliferation of Rat Synoviocytes Induced by IL-1β by Regulating Proliferation and Differentiation of T Lymphocytes. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-18.
https://search.emarefa.net/detail/BIM-1154919
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1154919
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر