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Src Is a Prime Target Inhibited by Celtis choseniana Methanol Extract in Its Anti-Inflammatory Action
المؤلفون المشاركون
Jeong, Deok
Yi, Young-Su
Kim, Han Gyung
Yoon, Deok Hyo
Kim, Jong-Hoon
Yoon, Keejung
Hong, Yo Han
Lee, Seungihm
Hong, Suntaek
Choi, Subin
Cho, Jae Youl
Sung, Gi-Ho
Lee, Jongsung
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-17، 17ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-03-14
دولة النشر
مصر
عدد الصفحات
17
التخصصات الرئيسية
الملخص EN
Celtis choseniana is the traditional plant used at Korea as a herbal medicine to ameliorate inflammatory responses.
Although Celtis choseniana has been traditionally used as a herbal medicine at Korea, no systemic research has been conducted on its anti-inflammatory activity.
Therefore, the present study explored an anti-inflammatory effect and its underlying molecular mechanism using Celtis choseniana methanol extract (Cc-ME) in macrophage-mediated inflammatory responses.
In vitro anti-inflammatory activity of Cc-ME was evaluated using RAW264.7 cells and peritoneal macrophages stimulated by lipopolysaccharide (LPS), pam3CSK4 (Pam3), or poly(I:C).
In vivo anti-inflammatory activity of Cc-ME was investigated using acute inflammatory disease mouse models, such as LPS-induced peritonitis and HCl/EtOH-induced gastritis.
The molecular mechanism of Cc-ME-mediated anti-inflammatory activity was examined by Western blot analysis and immunoprecipitation using whole cell and nuclear fraction prepared from the LPS-stimulated RAW264.7 cells and HEK293 cells.
Cc-ME inhibited NO production and mRNA expression of inducible nitric oxide synthase (iNOS), cyclooxygenase (COX-2), and tumor necrosis factor-alpha (TNF-α) in the RAW264.7 cells and peritoneal macrophages induced by LPS, pam3, or poly(I:C) without cytotoxicity.
High-performance liquid chromatography (HPLC) analysis showed that Cc-ME contained anti-inflammatory flavonoids quercetin, luteolin, and kaempferol.
Among those, the content of luteolin, which showed an inhibitory effect on NO production, was highest.
Cc-ME suppressed the NF-κB signaling pathway by targeting Src and interrupting molecular interactions between Src and p85, its downstream kinase.
Moreover, Cc-ME ameliorated the morphological finding of peritonitis and gastritis in the mouse disease models.
Therefore, these results suggest that Cc-ME exerted in vitro and in vivo anti-inflammatory activity in LPS-stimulated macrophages and mouse models of acute inflammatory diseases.
This anti-inflammatory activity of Cc-ME was dominantly mediated by targeting Src in NF-κB signaling pathway during macrophage-mediated inflammatory responses.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Kim, Han Gyung& Choi, Subin& Lee, Jongsung& Hong, Yo Han& Jeong, Deok& Yoon, Keejung…[et al.]. 2018. Src Is a Prime Target Inhibited by Celtis choseniana Methanol Extract in Its Anti-Inflammatory Action. Evidence-Based Complementary and Alternative Medicine،Vol. 2018, no. 2018, pp.1-17.
https://search.emarefa.net/detail/BIM-1155021
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Kim, Han Gyung…[et al.]. Src Is a Prime Target Inhibited by Celtis choseniana Methanol Extract in Its Anti-Inflammatory Action. Evidence-Based Complementary and Alternative Medicine No. 2018 (2018), pp.1-17.
https://search.emarefa.net/detail/BIM-1155021
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Kim, Han Gyung& Choi, Subin& Lee, Jongsung& Hong, Yo Han& Jeong, Deok& Yoon, Keejung…[et al.]. Src Is a Prime Target Inhibited by Celtis choseniana Methanol Extract in Its Anti-Inflammatory Action. Evidence-Based Complementary and Alternative Medicine. 2018. Vol. 2018, no. 2018, pp.1-17.
https://search.emarefa.net/detail/BIM-1155021
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1155021
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
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