Src Is a Prime Target Inhibited by Celtis choseniana Methanol Extract in Its Anti-Inflammatory Action
المؤلفون المشاركون
Jeong, Deok
Yi, Young-Su
Kim, Han Gyung
Yoon, Deok Hyo
Kim, Jong-Hoon
Yoon, Keejung
Hong, Yo Han
Lee, Seungihm
Hong, Suntaek
Choi, Subin
Cho, Jae Youl
Sung, Gi-Ho
Lee, Jongsung
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-17، 17ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-03-14
دولة النشر
مصر
عدد الصفحات
17
التخصصات الرئيسية
الملخص EN
Celtis choseniana is the traditional plant used at Korea as a herbal medicine to ameliorate inflammatory responses.
Although Celtis choseniana has been traditionally used as a herbal medicine at Korea, no systemic research has been conducted on its anti-inflammatory activity.
Therefore, the present study explored an anti-inflammatory effect and its underlying molecular mechanism using Celtis choseniana methanol extract (Cc-ME) in macrophage-mediated inflammatory responses.
In vitro anti-inflammatory activity of Cc-ME was evaluated using RAW264.7 cells and peritoneal macrophages stimulated by lipopolysaccharide (LPS), pam3CSK4 (Pam3), or poly(I:C).
In vivo anti-inflammatory activity of Cc-ME was investigated using acute inflammatory disease mouse models, such as LPS-induced peritonitis and HCl/EtOH-induced gastritis.
The molecular mechanism of Cc-ME-mediated anti-inflammatory activity was examined by Western blot analysis and immunoprecipitation using whole cell and nuclear fraction prepared from the LPS-stimulated RAW264.7 cells and HEK293 cells.
Cc-ME inhibited NO production and mRNA expression of inducible nitric oxide synthase (iNOS), cyclooxygenase (COX-2), and tumor necrosis factor-alpha (TNF-α) in the RAW264.7 cells and peritoneal macrophages induced by LPS, pam3, or poly(I:C) without cytotoxicity.
High-performance liquid chromatography (HPLC) analysis showed that Cc-ME contained anti-inflammatory flavonoids quercetin, luteolin, and kaempferol.
Among those, the content of luteolin, which showed an inhibitory effect on NO production, was highest.
Cc-ME suppressed the NF-κB signaling pathway by targeting Src and interrupting molecular interactions between Src and p85, its downstream kinase.
Moreover, Cc-ME ameliorated the morphological finding of peritonitis and gastritis in the mouse disease models.
Therefore, these results suggest that Cc-ME exerted in vitro and in vivo anti-inflammatory activity in LPS-stimulated macrophages and mouse models of acute inflammatory diseases.
This anti-inflammatory activity of Cc-ME was dominantly mediated by targeting Src in NF-κB signaling pathway during macrophage-mediated inflammatory responses.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Kim, Han Gyung& Choi, Subin& Lee, Jongsung& Hong, Yo Han& Jeong, Deok& Yoon, Keejung…[et al.]. 2018. Src Is a Prime Target Inhibited by Celtis choseniana Methanol Extract in Its Anti-Inflammatory Action. Evidence-Based Complementary and Alternative Medicine،Vol. 2018, no. 2018, pp.1-17.
https://search.emarefa.net/detail/BIM-1155021
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Kim, Han Gyung…[et al.]. Src Is a Prime Target Inhibited by Celtis choseniana Methanol Extract in Its Anti-Inflammatory Action. Evidence-Based Complementary and Alternative Medicine No. 2018 (2018), pp.1-17.
https://search.emarefa.net/detail/BIM-1155021
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Kim, Han Gyung& Choi, Subin& Lee, Jongsung& Hong, Yo Han& Jeong, Deok& Yoon, Keejung…[et al.]. Src Is a Prime Target Inhibited by Celtis choseniana Methanol Extract in Its Anti-Inflammatory Action. Evidence-Based Complementary and Alternative Medicine. 2018. Vol. 2018, no. 2018, pp.1-17.
https://search.emarefa.net/detail/BIM-1155021
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1155021
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر