Sedum sarmentosum Total Flavonoids Alleviate Schistosomiasis-Induced Liver Fibrosis by Altering TGF-β1 and Smad7 Expression
المؤلفون المشاركون
Yang, Peng-chun
Bai, Wei-zhe
Yan, Cai-hua
Huang, Wei-feng
Jiang, Shi-zhu
Wang, Jing
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-11-26
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Objectives.
Schistosomiasis is a parasitic disease that affects over 142 million people worldwide.
The main causes of death of schistosomiasis include liver granuloma and secondary hepatic cirrhosis resulting from severe fibrosis.
Despite intensive research, controlling liver fibrosis associated with schistosomiasis remains challenging.
Sedum sarmentosum total flavonoid (SSTF) is a promising agent to reduce liver fibrosis with an unknown mechanism.
Thus, the objectives of this study are to validate its effect on the liver fibrosis caused by schistosomiasis and to explore the underlying molecular mechanism.
Methods.
Sixty male Sprague-Dawley rats were randomly divided into six groups: one group of normal control and five groups of liver fibrosis induced by schistosomiasis japonica with or without SSTF or colchicine treatment, the latter serving as the positive control.
Liver tissues from each animal were harvested to observe the degree and grade of hepatic fibrosis.
We also measured the expression of transforming growth factor-beta 1 (TGF-β1) and Smad7 using RT-qPCR, Western blot, and immunohistochemistry.
Results.
Compared with the untreated model group, groups treated with SSTF at all three tested doses had significantly reduced hepatic fibrosis (P<0.05).
Each dose of SSTF also significantly reduced TGF-β1 protein expression and mRNA levels in the liver tissues (P<0.05).
In contrast, the middle and high doses of SSTF significantly increased Smad7 protein expression and mRNA levels (P<0.05).
Immunohistochemistry showed that each dose of SSTF reduced TGF-β1 protein expression (P<0.05).
Conclusion.
Our results demonstrated that SSTF alleviated schistosomiasis japonica-induced hepatic fibrosis by inhibiting the TGF-β1/Smad7 pathway.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Yang, Peng-chun& Bai, Wei-zhe& Wang, Jing& Yan, Cai-hua& Huang, Wei-feng& Jiang, Shi-zhu. 2020. Sedum sarmentosum Total Flavonoids Alleviate Schistosomiasis-Induced Liver Fibrosis by Altering TGF-β1 and Smad7 Expression. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1155075
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Yang, Peng-chun…[et al.]. Sedum sarmentosum Total Flavonoids Alleviate Schistosomiasis-Induced Liver Fibrosis by Altering TGF-β1 and Smad7 Expression. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1155075
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Yang, Peng-chun& Bai, Wei-zhe& Wang, Jing& Yan, Cai-hua& Huang, Wei-feng& Jiang, Shi-zhu. Sedum sarmentosum Total Flavonoids Alleviate Schistosomiasis-Induced Liver Fibrosis by Altering TGF-β1 and Smad7 Expression. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1155075
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1155075
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر