In Vitro Antidiabetic Activity and Mechanism of Action of Brachylaena elliptica (Thunb.) DC
المؤلفون المشاركون
Sagbo, Idowu Jonas
van de Venter, Maryna
Koekemoer, Trevor
Bradley, Graeme
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-07-04
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
In South Africa, the number of people suffering from diabetes is believed to be rising steadily and the current antidiabetic therapies are frequently reported to have adverse side effects.
Ethnomedicinal plant use has shown promise for the development of cheaper, cost-effective antidiabetic agents with fewer side effects.
The aim of this study was to investigate the antidiabetic activity and mechanism of action of aqueous leaf extract prepared from Brachylaena elliptica.
The potential of the extract for cytotoxicity was evaluated using MTT assay in HepG2 cells.
The effects of the plant extract on glucose utilization in HepG2 cells and L6 myotubes, triglyceride accumulation in 3T3-L1, INS-1 proliferation, glucose metabolism in INS-1 cells, and NO production in RAW macrophages were also investigated using cell culture procedures.
The inhibitory effects of the extract on the activities of different enzymes including alpha-amylase, alpha-glucosidase, pancreatic lipase, dipeptidyl peptidase IV (DPP-IV), collagenase, and CYP3A4 enzymes were evaluated.
The extract also tested against protein glycation using standard published procedure.
The plant extract displayed low level of toxicity, where both concentrations tested did not induce 50% cell death.
The extract caused a significant increase in glucose uptake in HepG2 liver cells, with efficacy significantly higher than the positive control, berberine.
The crude extract also displayed no significant effect on muscle glucose uptake, triglyceride accumulation in 3T3-L1, glucose metabolism in INS-1 cells, alpha-amylase, alpha-glucosidase, DPP-IV, lipase, protein glycation, and collagenase compared to the respective positive controls.
The extract displayed a proliferative effect on INS-1 cells at 25 μg/ml when compared to the negative control.
The plant also produced a concentration-dependent reduction in NO production in RAW macrophages and also demonstrated weak significant inhibition on CYP3A4 activity.
The findings provide evidence that B.
elliptica possess antidiabetic activity and appear to exact its hypoglycemic effect independent of insulin.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Sagbo, Idowu Jonas& van de Venter, Maryna& Koekemoer, Trevor& Bradley, Graeme. 2018. In Vitro Antidiabetic Activity and Mechanism of Action of Brachylaena elliptica (Thunb.) DC. Evidence-Based Complementary and Alternative Medicine،Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1155105
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Sagbo, Idowu Jonas…[et al.]. In Vitro Antidiabetic Activity and Mechanism of Action of Brachylaena elliptica (Thunb.) DC. Evidence-Based Complementary and Alternative Medicine No. 2018 (2018), pp.1-13.
https://search.emarefa.net/detail/BIM-1155105
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Sagbo, Idowu Jonas& van de Venter, Maryna& Koekemoer, Trevor& Bradley, Graeme. In Vitro Antidiabetic Activity and Mechanism of Action of Brachylaena elliptica (Thunb.) DC. Evidence-Based Complementary and Alternative Medicine. 2018. Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1155105
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1155105
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر