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A Validated LC-MSMS Method for Simultaneous Determination of Six Aconitum Alkaloids and Seven Ginsenosides in Rat Plasma and Application to Pharmacokinetics of Shen-Fu Prescription
المؤلفون المشاركون
Chang, Yanxu
He, Jun
Ouyang, Huizi
Chen, Xiaopeng
Liu, Fang
Tang, Zhidan
Bo, Fang
Liu, Huaming
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-06-28
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
A sensitive and reliable LC-MS/MS method has been developed and validated for simultaneous determination of six Aconitum alkaloids (aconitine, hypaconitine, mesaconitine, benzoylaconitine, benzoylhypacoitine, and benzoylmesaconine) and seven ginsenosides (Rb1, Rb2, Rc, Rd, Re, Rf, and Rg1) in rat plasma after oral administration of Shen-Fu prescription.
Psoralen was selected as internal standard (IS).
Protein precipitation with methanol was used in sample preparation.
The chromatographic separation was achieved on a CORTECS™ C18 column with 0.1% formic acid aqueous solution and acetonitrile as mobile phase.
The flow rate was 0.3 mL/min.
The detection was performed on a tandem mass system with an electrospray ionization (ESI) source in the positive ionization and multiple-reaction monitoring (MRM) mode.
The calibration curves of six Aconitum alkaloids and seven ginsenosides were linear over the range of 0.1-50 and 1-500 ng/mL, respectively.
The extraction recoveries of the analytes in plasma samples ranged from 64.2 to 94.1%.
Meanwhile, the intra- and interday precision of the analytes were less than 14.3%, and the accuracy was in the range of −14.2% to 9.8%.
The developed method was successfully applied to the pharmacokinetics of six Aconitum alkaloids and seven ginsenosides in rat plasma after oral administration of Shen-Fu prescription.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Ouyang, Huizi& Liu, Fang& Tang, Zhidan& Chen, Xiaopeng& Bo, Fang& Liu, Huaming…[et al.]. 2018. A Validated LC-MSMS Method for Simultaneous Determination of Six Aconitum Alkaloids and Seven Ginsenosides in Rat Plasma and Application to Pharmacokinetics of Shen-Fu Prescription. Evidence-Based Complementary and Alternative Medicine،Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1155410
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Ouyang, Huizi…[et al.]. A Validated LC-MSMS Method for Simultaneous Determination of Six Aconitum Alkaloids and Seven Ginsenosides in Rat Plasma and Application to Pharmacokinetics of Shen-Fu Prescription. Evidence-Based Complementary and Alternative Medicine No. 2018 (2018), pp.1-12.
https://search.emarefa.net/detail/BIM-1155410
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Ouyang, Huizi& Liu, Fang& Tang, Zhidan& Chen, Xiaopeng& Bo, Fang& Liu, Huaming…[et al.]. A Validated LC-MSMS Method for Simultaneous Determination of Six Aconitum Alkaloids and Seven Ginsenosides in Rat Plasma and Application to Pharmacokinetics of Shen-Fu Prescription. Evidence-Based Complementary and Alternative Medicine. 2018. Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1155410
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1155410
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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