Network Pharmacology-Based Approach to Revealing Biological Mechanisms of Qingkailing Injection against IschemicStroke: Focusing on Blood-Brain Barrier
المؤلفون المشاركون
Cheng, Fafeng
Wang, Xueqian
Ma, Chongyang
Zhang, Shuang
Fan, Shuning
Xu, Wenxiu
Jin, Na
Liu, Shuling
Lv, Kai
Wang, Qingguo
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-16، 16ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-08-27
دولة النشر
مصر
عدد الصفحات
16
التخصصات الرئيسية
الملخص EN
Ischemic stroke is the most common type of cerebrovascular accident worldwide.
It causes long-term disability and death.
Qingkailing (QKL) injection is a traditional Chinese patent medicine which has been clinically applied in the treatment of ischemic stroke for nearly thirty years.
In the present study, network pharmacology combined with experimentation was used to elucidate the mechanisms of QKL.
ADME screening and target prediction identified 62 active compounds and 275 targets for QKL.
Topological screening of the protein-protein interaction (PPI) network was used to build a core PPI network consisting of 408 nodes and 17,830 edges.
KEGG enrichment indicated that the main signaling pathway implicated in ischemic stroke involved hypoxia-inducible factor-1 (HIF-1).
Experimentation showed that QKL alleviated neurological deficits, brain infraction, blood-brain barrier (BBB) leakage, and tight junction degeneration in a mouse ischemic stroke model.
Two-photon laser scanning microscopy was used to evaluate BBB permeability and cerebral microvessel structure in living mice.
HIF-1α, matrix metalloproteinase-9 (MMP-9), and tight junction proteins such as occludin, zonula occludins-1 (ZO-1), claudin-5, and VE-Cadherin were measured by western blotting.
QKL upregulated ZO-1 and downregulated HIF-1α and MMP-9.
QKL has a multiapproach, multitarget, and synergistic effect against ischemic stroke.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Zhang, Shuang& Wang, Xueqian& Cheng, Fafeng& Ma, Chongyang& Fan, Shuning& Xu, Wenxiu…[et al.]. 2020. Network Pharmacology-Based Approach to Revealing Biological Mechanisms of Qingkailing Injection against IschemicStroke: Focusing on Blood-Brain Barrier. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-16.
https://search.emarefa.net/detail/BIM-1155476
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Zhang, Shuang…[et al.]. Network Pharmacology-Based Approach to Revealing Biological Mechanisms of Qingkailing Injection against IschemicStroke: Focusing on Blood-Brain Barrier. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-16.
https://search.emarefa.net/detail/BIM-1155476
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Zhang, Shuang& Wang, Xueqian& Cheng, Fafeng& Ma, Chongyang& Fan, Shuning& Xu, Wenxiu…[et al.]. Network Pharmacology-Based Approach to Revealing Biological Mechanisms of Qingkailing Injection against IschemicStroke: Focusing on Blood-Brain Barrier. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-16.
https://search.emarefa.net/detail/BIM-1155476
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1155476
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر