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The Role of Semipurified Fractions Isolated from Quercus infectoria on Bone Metabolism by Using hFOB 1.19 Human Fetal Osteoblast Cell Model
المؤلفون المشاركون
Abdullah, Amira Raudhah
Hapidin, Hermizi
Abdullah, Hasmah
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-05-13
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
Background.
Quercus infectoria (QI) is a plant used in traditional medicines in Asia.
The plant was reported to contain various active phytochemical compounds that have potential to stimulate bone formation.
However, the precise mechanism of the stimulation effect of QI on osteoblast has not been elucidated.
The present study was carried out to isolate QI semipurified fractions from aqueous QI extract and to delineate the molecular mechanism of QI semipurified fraction that enhanced bone formation by using hFOB1.19 human fetal osteoblast cell model.
Methods.
Isolation of QI semipurified fractions was established by means of column chromatography and thin layer chromatography.
Established QI semipurified fractions were identified using Liquid Chromatography-Mass Spectrometry (LC-MS).
Cells were treated with derived QI semipurified fractions and investigated for mineralization deposition and protein expression level of BMP-2, Runx2, and OPN by ELISA followed gene expression analysis of BMP-2 and Runx2 by RT-PCR.
Results.
Column chromatography isolation and purification yield Fractions A, B, and C.
LC-MS analysis reveals the presence of polyphenols in each fraction.
Results show that QI semipurified fractions increased the activity and upregulated the gene expression of BMP-2 and Runx2 at day 1, day 3, and day 7.
OPN activity increased in cells treated with QI semipurified fractions at day 1 and day 3.
Meanwhile, at day 7, expression of OPN decreased in activity.
Furthermore, the study showed that combination of Fractions A, B, and C with osteoporotic drug (pamidronate) further increased the activity and upregulated the gene expression of BMP-2 and Runx2.
Conclusions.
These findings demonstrated that polyphenols from semipurified fractions of QI enhanced bone formation through expression of the investigated bone-related marker that is its potential role when combined with readily available osteoporotic drug.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Abdullah, Amira Raudhah& Hapidin, Hermizi& Abdullah, Hasmah. 2018. The Role of Semipurified Fractions Isolated from Quercus infectoria on Bone Metabolism by Using hFOB 1.19 Human Fetal Osteoblast Cell Model. Evidence-Based Complementary and Alternative Medicine،Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1155492
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Abdullah, Amira Raudhah…[et al.]. The Role of Semipurified Fractions Isolated from Quercus infectoria on Bone Metabolism by Using hFOB 1.19 Human Fetal Osteoblast Cell Model. Evidence-Based Complementary and Alternative Medicine No. 2018 (2018), pp.1-13.
https://search.emarefa.net/detail/BIM-1155492
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Abdullah, Amira Raudhah& Hapidin, Hermizi& Abdullah, Hasmah. The Role of Semipurified Fractions Isolated from Quercus infectoria on Bone Metabolism by Using hFOB 1.19 Human Fetal Osteoblast Cell Model. Evidence-Based Complementary and Alternative Medicine. 2018. Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1155492
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1155492
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
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