Gubenzhike Recipe Ameliorates Respiratory Mucosal Immunity in Mice with Chronic Obstructive Pulmonary Disease through Upregulation of the γδT Lymphocytes and KGF Levels
المؤلفون المشاركون
Cai, Zhe
Wang, Yue-qi
Liao, Qiang
Tang, Shi-huan
Zhang, Hongchun
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-20، 20ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-03-24
دولة النشر
مصر
عدد الصفحات
20
التخصصات الرئيسية
الملخص EN
Background.
Gubenzhike recipe, a traditional Chinese herbal compound, was assumed to have a possible beneficial effect on COPD.
This study was designed to elucidate the mechanism from the perspective of respiratory mucosal immunity.
Methods.
COPD model was induced by exposure to cigarette smoke and LPS instillation in mice for 12 weeks.
Animals were administered solution of Gubenzhike recipe by intragastric gavage daily for 4 weeks.
After that, mice were sacrificed for lung function test and histological examination of lung tissues.
The levels of IL-6 and IL-13 in serum, bronchoalveolar lavage fluid (BALF), and intestinal mucus were measured by ELISA.
The KGF and KGFR in lung tissue were analysed by immunohistochemical staining, ELISA, and western blotting, and the mRNA expressions were assessed by PCR.
γδT lymphocytes in the lungs were isolated and analysed by immunohistochemical staining and flow cytometry.
Results.
Gubenzhike recipe improved the structure of airway and damage of lung tissue and also the respiratory status and lung function, reduced the content of IL-6 in serum and BALF and IL-13 in BALF and intestinal mucus, increased the proportion of γδT cells in lung tissue, and promoted the secretion of KGF and KGFR (P<0.05).
Conclusion.
We for the first time demonstrated an experimental procedure for the isolation of γδT lymphocytes from lung tissue.
This study suggested that Gubenzhike recipe could enhance the respiratory mucosal immunity which provided experimental evidence for its effects of reinforcing “wei qi” by means of strengthening vital qi, tonifying spleen and kidney, relieving cough, and reducing phlegm in TCM.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Wang, Yue-qi& Liao, Qiang& Tang, Shi-huan& Cai, Zhe& Zhang, Hongchun. 2020. Gubenzhike Recipe Ameliorates Respiratory Mucosal Immunity in Mice with Chronic Obstructive Pulmonary Disease through Upregulation of the γδT Lymphocytes and KGF Levels. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-20.
https://search.emarefa.net/detail/BIM-1155536
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Wang, Yue-qi…[et al.]. Gubenzhike Recipe Ameliorates Respiratory Mucosal Immunity in Mice with Chronic Obstructive Pulmonary Disease through Upregulation of the γδT Lymphocytes and KGF Levels. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-20.
https://search.emarefa.net/detail/BIM-1155536
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Wang, Yue-qi& Liao, Qiang& Tang, Shi-huan& Cai, Zhe& Zhang, Hongchun. Gubenzhike Recipe Ameliorates Respiratory Mucosal Immunity in Mice with Chronic Obstructive Pulmonary Disease through Upregulation of the γδT Lymphocytes and KGF Levels. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-20.
https://search.emarefa.net/detail/BIM-1155536
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1155536
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر