Profiling of the Causative Bacteria in Infected Lymphocysts after Lymphadenectomy for Gynecologic Cancer by Pyrosequencing the 16S Ribosomal RNA Gene Using Next-Generation Sequencing Technology
المؤلفون المشاركون
Nogami, Yuya
Adachi, Masataka
Tominaga, Eiichiro
Banno, Kouji
Kobayashi, Yusuke
Irie, Haruko
Aoki, Daisuke
المصدر
Infectious Diseases in Obstetrics and Gynecology
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-5، 5ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-02-21
دولة النشر
مصر
عدد الصفحات
5
التخصصات الرئيسية
الملخص EN
Background.
Surgery for gynecologic cancer with lymphadenectomy and pelvic radiotherapy can produce lymphoceles that sometimes complicate with infection, resulting in abscesses.
The true pathogenic bacteria of abscesses are not always found because of false-negative results due to administered antibiotics and difficulty with detection, including for anaerobic bacteria.
Analyzing bacteria flora by next-generation sequencing (NGS) using 16S ribosomal DNA may reveal the true pathogenic bacteria in abscesses.
This is the first report on causative pathogens for infectious lymphocele using this technology.
Methods.
The subjects were patients who developed infectious lymphocele after surgery for gynecologic cancer at our hospital from July 2015 to September 2016.
NGS analyses of bacterial flora were performed using specimens preserved at -80°C.
Two steps of PCR were performed for purified DNA samples to obtain sequence libraries.
Processing of sequence data, including operational taxonomic unit (OTU) definition, taxonomy assignment, and an OTU BLAST search were performed.
All patients gave written informed consent and the study was approved by the institutional research ethics committee.
Results.
Six patients underwent puncture and drainage.
The result in most cases indicated a single causative pathogen, including Staphylococcus lugdunensis, Streptococcus dysgalactiae, Streptococcus equinus, Enterococcus saccharolyticus, and Escherichia coli.
Conclusions.
NGS revealed that the causative bacteria in lymphocele infection are normally a single strain, such as a surface Gram-positive coccus or enteric bacteria.
Antibiotics should be chosen as appropriate for elimination of these respective bacteria.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Nogami, Yuya& Banno, Kouji& Adachi, Masataka& Irie, Haruko& Kobayashi, Yusuke& Tominaga, Eiichiro…[et al.]. 2019. Profiling of the Causative Bacteria in Infected Lymphocysts after Lymphadenectomy for Gynecologic Cancer by Pyrosequencing the 16S Ribosomal RNA Gene Using Next-Generation Sequencing Technology. Infectious Diseases in Obstetrics and Gynecology،Vol. 2019, no. 2019, pp.1-5.
https://search.emarefa.net/detail/BIM-1155712
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Nogami, Yuya…[et al.]. Profiling of the Causative Bacteria in Infected Lymphocysts after Lymphadenectomy for Gynecologic Cancer by Pyrosequencing the 16S Ribosomal RNA Gene Using Next-Generation Sequencing Technology. Infectious Diseases in Obstetrics and Gynecology No. 2019 (2019), pp.1-5.
https://search.emarefa.net/detail/BIM-1155712
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Nogami, Yuya& Banno, Kouji& Adachi, Masataka& Irie, Haruko& Kobayashi, Yusuke& Tominaga, Eiichiro…[et al.]. Profiling of the Causative Bacteria in Infected Lymphocysts after Lymphadenectomy for Gynecologic Cancer by Pyrosequencing the 16S Ribosomal RNA Gene Using Next-Generation Sequencing Technology. Infectious Diseases in Obstetrics and Gynecology. 2019. Vol. 2019, no. 2019, pp.1-5.
https://search.emarefa.net/detail/BIM-1155712
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1155712
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر