Chromatographic Fingerprint and Quantitative Analysis of Commercial Pheretima aspergillum (Guang Dilong) and Its Adulterants by UPLC-DAD
المؤلفون المشاركون
Ma, Zhi-guo
Sun, Jie
Tian, Fang
Zhang, Ying
Wu, Menghua
Mao, Runqian
Le, Zhiyong
Xu, Dongjin
Cao, Hui
المصدر
International Journal of Analytical Chemistry
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-01-09
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Guang Dilong is a Traditional Chinese Medicine prepared from the dried body of Pheretima aspergillum (E.
Perrier), a species of earthworm.
However, preparations of Guang Dilong may be adulterated by other species and a method of quality control is needed.
A method was developed to analyze and authenticate commercial Guang Dilong, utilizing ultra-high performance liquid chromatography (UHPLC) coupled with diode array detection (DAD).
Equipment included an Acquity UPLC HSS T3 column (100 mm × 2.1 mm, 1.8 μm).
The mobile phase consisted of acetonitrile and 0.01% formic acid, pumped at 0.3 mL/min.
Wavelength detection was at 260 nm.
Twenty-two batches of confirmed P.
aspergillum samples (reference) from different sources and 20 batches of adulterated samples were analyzed to establish a reference fingerprint for commercial Guang Dilong.
Five peaks in the fingerprints of the reference batches were identified as characteristic; six characteristic peaks in the fingerprints of the adulterants were identified by comparing their retention time with those of the references.
The total 42 batches of samples were compared with the reference fingerprint, and the fingerprints of the P.
aspergillum samples were similar.
The UHPLC-DAD method can simultaneously determine the contents of six compounds (hypoxanthine, xanthine, uridine, inosine, guanosine, and adenosine) in the reference and adulterated batches.
The six compounds showed good regression (r > 0.9999) within test ranges.
The recovery (accuracy) was 98.25 to 101.68%, with relative standard deviation <2.67%.
In summary, this UHPLC-DAD method combines chromatographic fingerprint with quantification analysis and could be readily used as an efficient quality control method for Guang Dilong.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Sun, Jie& Tian, Fang& Zhang, Ying& Wu, Menghua& Mao, Runqian& Le, Zhiyong…[et al.]. 2019. Chromatographic Fingerprint and Quantitative Analysis of Commercial Pheretima aspergillum (Guang Dilong) and Its Adulterants by UPLC-DAD. International Journal of Analytical Chemistry،Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1155880
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Sun, Jie…[et al.]. Chromatographic Fingerprint and Quantitative Analysis of Commercial Pheretima aspergillum (Guang Dilong) and Its Adulterants by UPLC-DAD. International Journal of Analytical Chemistry No. 2019 (2019), pp.1-10.
https://search.emarefa.net/detail/BIM-1155880
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Sun, Jie& Tian, Fang& Zhang, Ying& Wu, Menghua& Mao, Runqian& Le, Zhiyong…[et al.]. Chromatographic Fingerprint and Quantitative Analysis of Commercial Pheretima aspergillum (Guang Dilong) and Its Adulterants by UPLC-DAD. International Journal of Analytical Chemistry. 2019. Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1155880
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1155880
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر