Application of Factorial and Doehlert Designs for the Optimization of the Simultaneous Separation and Determination of Antimigraine Drugs in Pharmaceutical Formulations by RP-HPLC-UV
المؤلفون المشاركون
Jebali, Sami
Belgacem, Chaouki
Louhaichi, Mohamed Radhouen
Bahri, Senda
Latrous El Atarche, Latifa
المصدر
International Journal of Analytical Chemistry
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-08-15
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
A sensitive, precise, accurate, and specific isocratic reversed-phase high-performance liquid chromatographic (RP-HPLC) method for the simultaneous separation and determination of zolmitriptan, naratriptan, dihydroergotamine, ketotifen, and pizotifen in pharmaceutical formulations has been developed and validated.
An experimental design was applied for the optimization of the chromatographic parameters.
A two-level full factorial 2k was used for studying the interaction between the variables to be optimized: the percentage of acetonitrile in the mobile phase, mobile-phase pH, nature of the buffer, and column oven temperature.
The most significant parameters are the percentage of acetonitrile and the mobile-phase pH.
These significant parameters were optimized using the Doehlert matrix.
The optimum separation was achieved by means of a Waters XBridge C18 column (250 mm × 4.6 mm, 5 μm) with a mobile phase consisting of acetonitrile and a 10 mM sodium perchlorate buffer (38 : 62, v/v) at a flow rate of 1.0 mL·min−1 and UV detection at 220 nm.
The selectivity, method linearity, accuracy, and precision were examined as part of the method validation.
The described method shows excellent linearity over a range of 30 to 70 μg·mL−1 for all compounds with correlation coefficients higher than 0.995.
The standard deviations of the intraday and interday precision were between 0.75 and 1.94%.
The validated method was successfully applied to perform routine analysis of these compounds in different pharmaceutical products such as syrups and tablets.
In the presence of some preservatives, it was found that there were no peaks at the related peak locations.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Jebali, Sami& Belgacem, Chaouki& Louhaichi, Mohamed Radhouen& Bahri, Senda& Latrous El Atarche, Latifa. 2019. Application of Factorial and Doehlert Designs for the Optimization of the Simultaneous Separation and Determination of Antimigraine Drugs in Pharmaceutical Formulations by RP-HPLC-UV. International Journal of Analytical Chemistry،Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1156016
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Jebali, Sami…[et al.]. Application of Factorial and Doehlert Designs for the Optimization of the Simultaneous Separation and Determination of Antimigraine Drugs in Pharmaceutical Formulations by RP-HPLC-UV. International Journal of Analytical Chemistry No. 2019 (2019), pp.1-11.
https://search.emarefa.net/detail/BIM-1156016
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Jebali, Sami& Belgacem, Chaouki& Louhaichi, Mohamed Radhouen& Bahri, Senda& Latrous El Atarche, Latifa. Application of Factorial and Doehlert Designs for the Optimization of the Simultaneous Separation and Determination of Antimigraine Drugs in Pharmaceutical Formulations by RP-HPLC-UV. International Journal of Analytical Chemistry. 2019. Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1156016
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1156016
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر