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Cytotoxic Constituents of the Bark of Hypericum roeperianum towards Multidrug-Resistant Cancer Cells
المؤلفون المشاركون
Kuete, Victor
Tankeo, Simplice B.
Mbaveng, Armelle T.
Damen, Francois
Simo Mpetga, James D.
Guefack, Michel-Gael F.
Bitchagno, Gabin T. M.
Çelik, İlhami
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-09-25
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
The global cancer burden remains a serious concern with the alarming incidence of one in eight men and one in eleven women dying in developing countries.
This situation is aggravated by the multidrug resistance (MDR) of cancer cells that hampers chemotherapy.
In this study, the cytotoxicity of the methanol extract (HRB), fractions (HRBa, HRBb, and HRBa1-5), and compounds from the bark of Hypericum roeperianum (HRB) was evaluated towards a panel of 9 cancer cell lines.
The mode of action of the HRB and trichadonic acid (1) was also studied.
Column chromatography was applied to isolate the constituents of HRB.
The cytotoxicity of botanicals and phytochemicals was evaluated by the resazurin reduction assay (RRA).
Caspase-Glo assay was used to evaluate the activity of caspases, and reactive oxygen species (ROS) (H2DCFH-DA) were assessed by flow cytometry.
Phytochemicals isolated from HRB were trichadonic acid (1), fridelan-3-one (2), 2-hydroxy-5-methoxyxanthone (3), norathyriol (4), 1,3,5,6-tetrahydroxyxanthone (5), betulinic acid (6), 3′-hydroxymethyl-2′-(4″-hydroxy-3″,5″-dimethoxyphenyl)-5′,6′:5,6-(6,8-dihydroxyxanthone)-1′,4′-dioxane (7), and 3′-hydroxymethyl-2′-(4″-hydroxy-3″,5″-dimethoxyphenyl)-5′,6′:5,6-(xanthone)-1′,4′-dioxane (8).
Botanicals HRB, HRBa, HRBa2-4, HRBb, and doxorubicin displayed cytotoxic effects towards the 9 tested cancer cell lines.
The recorded IC50 values ranged from 11.43 µg/mL (against the P-glycoprotein (gp)-overexpressing CEM/ADR5000 leukemia cells) to 26.75 µg/mL (against HCT116 (p53+/+) colon adenocarcinoma cells) for the crude extract HRB.
Compounds 1, 5, and doxorubicin displayed cytotoxic effects towards the 9 tested cancer cell lines with IC50 values varying from 14.44 µM (against CCRF-CEM leukemia cells) to 44.20 µM (against the resistant HCT116 (p53−/−) cells) for 1 and from 38.46 µM (against CEM/ADR5000 cells) to 112.27 µM (against the resistant HCT116 (p53−/−) cells) for 5.
HRB and compound 1 induced apoptosis in CCRF-CEM cells.
The apoptotic process was mediated by enhanced ROS production for HRB or via caspases activation and enhanced ROS production for compound 1.
This study demonstrated that Hypericum roeperianum is a potential source of cytotoxic phytochemicals such as trichadonic acid and could be further exploited in cancer chemotherapy.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Guefack, Michel-Gael F.& Damen, Francois& Mbaveng, Armelle T.& Tankeo, Simplice B.& Bitchagno, Gabin T. M.& Çelik, İlhami…[et al.]. 2020. Cytotoxic Constituents of the Bark of Hypericum roeperianum towards Multidrug-Resistant Cancer Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1156100
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Guefack, Michel-Gael F.…[et al.]. Cytotoxic Constituents of the Bark of Hypericum roeperianum towards Multidrug-Resistant Cancer Cells. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-11.
https://search.emarefa.net/detail/BIM-1156100
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Guefack, Michel-Gael F.& Damen, Francois& Mbaveng, Armelle T.& Tankeo, Simplice B.& Bitchagno, Gabin T. M.& Çelik, İlhami…[et al.]. Cytotoxic Constituents of the Bark of Hypericum roeperianum towards Multidrug-Resistant Cancer Cells. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1156100
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1156100
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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