Pulchinenosides from Pulsatilla Chinensis Increase P-Glycoprotein Activity and Induce P-Glycoprotein Expression
المؤلفون المشاركون
Liu, Yali
Su, Dan
Zhang, Ling
Gerk, Phillip M.
Wei, Shaofeng
Zang, Zhenzhong
Wang, Meng
Cai, Jinyang
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-02-19
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Five pulchinenosides (pulchinenoside B3, pulchinenoside BD, pulchinenoside B7, pulchinenoside B10, and pulchinenoside B11) isolated from Pulsatilla chinensis (Bge) Regel saponins extract exhibited strong antitumor activities but poor gastrointestinal absorption properties.
The enteric induction of P-glycoprotein (P-gp) is understood to restrict the oral bioavailability of some pharmaceutical compounds and lead to adverse drug reactions.
Therefore, the present investigation was intended to delineate the impacts of pulchinenosides on cellular P-gp function and expression using Sf9 membrane vesicles and LS180 cells as a surrogate of human intestinal epithelial cells.
Preliminary cytotoxic studies showed that 10 μM was an acceptable concentration for cytotoxicity and antiproliferation studies for all pulchinenosides using the alamarBlue assay.
The cell cycle of LS180 cells detected by flow cytometry was not significantly influenced after 48 hours of coincubation with 10 μM of pulchinenosides.
In the presence of pulchinenosides, the ATP-dependent transport of N-methyl-quinidine mediated by P-glycoprotein was stimulated significantly.
The upregulation of P-glycoprotein and mRNA levels was found by Western blot and real-time PCR analysis in LS180 cells.
Parallel changes indicate that all pulchinenosides are exposed to pulchinenosides-mediated transcriptional regulation.
In conclusion, pulchinenosides could induce P-glycoprotein expression and directly increase its functional activity.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Liu, Yali& Zhang, Ling& Wei, Shaofeng& Cai, Jinyang& Zang, Zhenzhong& Wang, Meng…[et al.]. 2020. Pulchinenosides from Pulsatilla Chinensis Increase P-Glycoprotein Activity and Induce P-Glycoprotein Expression. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1156297
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Liu, Yali…[et al.]. Pulchinenosides from Pulsatilla Chinensis Increase P-Glycoprotein Activity and Induce P-Glycoprotein Expression. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-8.
https://search.emarefa.net/detail/BIM-1156297
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Liu, Yali& Zhang, Ling& Wei, Shaofeng& Cai, Jinyang& Zang, Zhenzhong& Wang, Meng…[et al.]. Pulchinenosides from Pulsatilla Chinensis Increase P-Glycoprotein Activity and Induce P-Glycoprotein Expression. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1156297
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1156297
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر