Differences in MicroRNA Expression in Chronic Hepatitis B Patients with Early Liver Fibrosis Based on Traditional Chinese Medicine Syndromes
المؤلفون المشاركون
Zhao, Peng-Tao
Shi, Mei-Jie
Xiao, Huan-Ming
Xie, Yu-Bao
Cai, Gao-Shu
Li, Sheng
Jiang, Jun-Min
Li, Ying-Xian
Zhang, Chao-Zhen
Cao, Min-Ling
Chen, Qu-Bo
Tan, Zhi-Jian
Gao, Heng-Jun
Chi, Xiao-Ling
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-10-31
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
The aim of this study was to determine if microRNA (miRNA) expression is different among chronic hepatitis B (CHB) patients with early liver fibrosis classified according to traditional Chinese medicine (TCM) syndromes.
Eighteen CHB-fibrosis patients and 12 CHB patients without fibrosis were enrolled.
The CHB-fibrosis group included 9 patients with the TCM syndrome of Ganyu Pixu Xueyu (GYPXXY), characterized by liver stagnation, spleen deficiency, and blood stasis, and 9 patients with the TCM syndrome of Qixu Xueyu (QXXY), characterized by deficiency of qi, blood, and blood stasis.
Agilent miRNA microarray was performed first in liver specimens to determine whether miRNA expression is different in patients with these two TCM syndromes of CHB-fibrosis.
Gene Ontology (GO) analysis and KEGG analysis were applied to determine the roles of the differentially expressed miRNAs.
QRT-PCR was performed to validate the Agilent miRNA microarray results.
Compared with GYPXXY patients, 6 differentially expressed miRNAs were upregulated (miR-144-5p, miR-18a-5p, miR-148b-3p, miR-654-3p, miR-139-3p, and miR-24-1-5p) and 1 was downregulated (miR-6834-3p) in QXXY patients.
According to qRT-PCR data, miR-144-5p and miR-654-3p were confirmed as upregulated in CHB-liver fibrosis patients compared to CHB patients without fibrosis, whereas the other 4 miRNAs were not significantly different.
More importantly, miR-654-3p was confirmed to be significantly upregulated in QXXY patients compared with values in GYPXXY patients, whereas no significant difference was found in miR-144-5p.
Moreover, the pathways of central carbon metabolism in cancer and cell cycle related to miR-654-3p and the target genes of PTEN and ATM were found to be different between QXXY patients and GYPXXY patients.
These results indicate that there are different miRNAs, pathways, and target genes between QXXY patients and GYPXXY patients.
However, due to the limited sample, whether miR-654-3p and the target genes PTEN and ATM could be molecular markers to differentiate TCM syndromes could not be established.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Shi, Mei-Jie& Xiao, Huan-Ming& Xie, Yu-Bao& Jiang, Jun-Min& Zhao, Peng-Tao& Cai, Gao-Shu…[et al.]. 2020. Differences in MicroRNA Expression in Chronic Hepatitis B Patients with Early Liver Fibrosis Based on Traditional Chinese Medicine Syndromes. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1156799
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Shi, Mei-Jie…[et al.]. Differences in MicroRNA Expression in Chronic Hepatitis B Patients with Early Liver Fibrosis Based on Traditional Chinese Medicine Syndromes. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1156799
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Shi, Mei-Jie& Xiao, Huan-Ming& Xie, Yu-Bao& Jiang, Jun-Min& Zhao, Peng-Tao& Cai, Gao-Shu…[et al.]. Differences in MicroRNA Expression in Chronic Hepatitis B Patients with Early Liver Fibrosis Based on Traditional Chinese Medicine Syndromes. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1156799
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1156799
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر