Understanding the Role of Gui-Zhi-Fu-Ling-Capsules (Chinese Medicine) for Treatment of Endometriosis in the Rat Model: Using NMR Based Metabolomics
المؤلفون المشاركون
Zhou, Jue
Ding, Zhi-Ming
Hardiman, Paul J.
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-12-19
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
The objective of this study is to identify the changes of metabolites in the rat endometriosis models treated with Gui-Zhi-Fu-Ling-capsules (GZFLC), a classic Chinese medicinal formula, and to explore the effects of GZFLC on the serum levels of transforming growth factor-β1 (TGF-β1) and the mRNA expression levels of vascular endothelial growth factor (VEGF) and glucose transporter 4 (GLUT-4) in the endometriotic tissues.
Forty female Wistar rats were randomly divided into the sham-operation group (Normal group), Model group, Danazol group, and GZFLC group.
The serum levels of TGF-β1 were measured using enzyme-linked immune-sorbent assay (ELISA).
The mRNA expression levels of VEGF and GLUT-4 in the endometriotic tissue of the rat endometriosis models were measured using real-time quantitative PCR.
The metabolites in urine were detected by 1H NMR method.
Eight identified metabolites of the NMR resonance were involved in the glycolysis metabolism.
Among the 8 metabolites, Lactate, Acetate, TMA, and Formate were downregulated with GZFLC.
Citrate, TMAO, Taurine, and Hippurate were unregulated with GZFLC.
The serum levels of TGF-β1 in the Danazol and GZFLC groups were significantly higher than those of Normal group and significantly lower than the Model group.
GZFLC treatment significantly decreased the GLUT-4 and VEGF mRNA expression levels in the endometriotic tissues of the endometriosis rats (P < 0.05).
GZFLC significantly decreased the GLUT-4 mRNA expression levels in rats of GZFLC group compared with Danazol group.
It is through regulating the metabolites changes of glycolysis or gluconeogenesis that GZFLC significantly affected the expression levels of TGF-β1, GLUT-4, and VEGF of the model rats with endometriosis.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Zhou, Jue& Ding, Zhi-Ming& Hardiman, Paul J.. 2018. Understanding the Role of Gui-Zhi-Fu-Ling-Capsules (Chinese Medicine) for Treatment of Endometriosis in the Rat Model: Using NMR Based Metabolomics. Evidence-Based Complementary and Alternative Medicine،Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1156985
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Zhou, Jue…[et al.]. Understanding the Role of Gui-Zhi-Fu-Ling-Capsules (Chinese Medicine) for Treatment of Endometriosis in the Rat Model: Using NMR Based Metabolomics. Evidence-Based Complementary and Alternative Medicine No. 2018 (2018), pp.1-9.
https://search.emarefa.net/detail/BIM-1156985
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Zhou, Jue& Ding, Zhi-Ming& Hardiman, Paul J.. Understanding the Role of Gui-Zhi-Fu-Ling-Capsules (Chinese Medicine) for Treatment of Endometriosis in the Rat Model: Using NMR Based Metabolomics. Evidence-Based Complementary and Alternative Medicine. 2018. Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1156985
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1156985
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر