Antiadipogenesis of Oroxylum indicum (L.) Kurz Extract via PPARγ2 in 3T3-L1 Adipocytes
المؤلفون المشاركون
Eumkeb, Griangsak
Hengpratom, Tanaporn
Lowe, Gordon Matthew
Ngernsoungnern, Apichart
Ngernsoungnern, Piyada
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-01-07
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Oroxylum indicum is regarded as a traditional food with medicinal properties and is used widely throughout Asia.
It has previously been demonstrated that O.
indicum extract (OIE) was able to suppress the differentiation of 3T3-L1 preadipocytes to adipocytes.
However, the mechanism underlying the antiadipogenesis of this plant has not been fully investigated.
The present study aimed to explore the impact of OIE at 50 to 200 μg mL−1 on the molecular mechanism involved in the antiadipogenic activity in 3T3-L1 cells at day 0 of their differentiation to adipocytes.
The morphology and biochemistry of the cells on day 12 were investigated and compared to the relevant controls.
Adiponectin was measured using enzyme-linked immunosorbent assay (ELISA).
The mRNA expression of peroxisome proliferator-activated receptor-gamma 2 (PPARγ2), sterol regulatory element-binding proteins 1c (SREBP-1c), fatty acid synthetase (FAS), glucose transporter (GLUT4), and leptin in adipocytes was determined by real-time PCR.
The results demonstrated that the OIE at 200 μg mL−1 exhibited strongest suppression on intracellular lipid accumulation.
The levels of adiponectin were dramatically increased in the untreated adipocytes, whereas significantly decreased in the 200 μg mL−1 OIE-treated adipocytes (P<0.05).
Expression of the mRNAs revealed that OIE-treated adipocytes at 200 μg mL−1 significantly inhibited the expression of PPARγ2 and SREBP-1c and lowered the level of expression of GLUT4, FAS, and leptin compared to the control (P<0.05).
These findings suggest that OIE inhibits adipocyte differentiation along with the downregulation of PPARγ2, SREBP-1c, and GLUT4, leading to the decrease in the expression of FAS and adipokine (leptin and adiponectin).
Thus, OIE might be developed for hyperlipidemia and obesity prevention.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Hengpratom, Tanaporn& Ngernsoungnern, Apichart& Ngernsoungnern, Piyada& Lowe, Gordon Matthew& Eumkeb, Griangsak. 2020. Antiadipogenesis of Oroxylum indicum (L.) Kurz Extract via PPARγ2 in 3T3-L1 Adipocytes. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1157048
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Hengpratom, Tanaporn…[et al.]. Antiadipogenesis of Oroxylum indicum (L.) Kurz Extract via PPARγ2 in 3T3-L1 Adipocytes. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1157048
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Hengpratom, Tanaporn& Ngernsoungnern, Apichart& Ngernsoungnern, Piyada& Lowe, Gordon Matthew& Eumkeb, Griangsak. Antiadipogenesis of Oroxylum indicum (L.) Kurz Extract via PPARγ2 in 3T3-L1 Adipocytes. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1157048
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1157048
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر